Abstract：
Reticulitermes chinensis Snyder is a serious pest in China, and the odorant receptor co-receptor gene RcOrco plays a crucial role in olfaction. However, the function of RcOrco in the resistance of termites to entomopathogens has not been reported. We constructed dsRcOrco-HT115 engineered bacteria based on the RcOrco sequence from the full-length transcriptome data of R. chinensis. The engineered bacteria expressed dsRNA of RcOrco. Sonication was used to inactivate the dsRNA-HT115 strain and obtain a large amount of dsRcOrco. The dsRcOrco produced using this method overcame the problem that genetically engineered bacteria could not be applied directly and improved its effectiveness against termites. Bioassays using the dsRcOrco generated using this method showed that dsRcOrco significantly increased the toxicity of the bacterial and fungal pathogens to R. chinensis. The present study showed, for the first time, the function of Orco in termite resistance to pathogens, and the results provide a theoretical basis for the development and application of termite RNA biopesticides.
摘要：
中国网虫是中国的一种严重害虫，气味受体共受体基因RcOrco在嗅觉中起着至关重要的作用。然而,尚未报道RcOrco在白蚁对昆虫病原体的抗性中的功能。我们基于来自R.chinensis全长转录组数据的RcOrco序列构建了dsRcOrco-HT115工程化细菌。工程化细菌表达RcOrco的dsRNA。超声处理用于灭活dsRNA-HT115菌株并获得大量dsRcOrco。使用这种方法生产的dsRcOrco克服了基因工程细菌不能直接应用的问题，并提高了其对白蚁的有效性。使用使用该方法产生的dsRcOrco的生物测定显示,dsRcOrco显著增加了细菌和真菌病原体对R.chinensis的毒性。目前的研究表明,第一次,Orco在白蚁对病原体的抗性中的功能，研究结果为白蚁RNA生物农药的开发和应用提供了理论依据。
