Abstract：
Mao Jian Tea (MJT) has been generally consumed as a digestive aid for more than a hundred years in the Shanxi province of China. However, determination of its efficacy still remains elusive. This study investigated the effect of Mao Jian Green Tea (MJGT) on gastrointestinal motility. The biphasic effects of the hydro extracts of MJGT on gastric emptying and small intestinal propulsion of rats were identified in vivo; namely, the low (MJGT_L) and medium (MJGT_M) concentrations promoted gastrointestinal motility (p < 0.05), whereas the high concentration (MJGT_H) showed the opposite effect (p < 0.01). The expression levels of the gastric hormones, GAS, MTL and VIP (p < 0.05) were consistent with the gastrointestinal motility variation, with the exception of MTL in MJGT_H group (p > 0.01). Two flavonoids, eriodictyol (0.152 mg/mL) and luteolin (0.034 mg/mL), and the corresponding glycosides eriodictyol-7-O-glucoside (0.637 mg/mL) and luteolin-7-O-glucoside (0.216 mg/mL), dominated the hydro extracts identified by HPLC and UPLC-ESI-MS. These compounds can regulate the muscle strip contractions isolated from the gastrointestinal tissues. Additionally, the different concentrations also influenced the gut microbiota accordingly characterized by 16S rDNA gene sequencing. The MJGT_L boosted several probiotic bacteria, such as Muribaculaceae (1.77-fold), Prevotellaceae (1.85-fold) and Lactobacillaceae (2.47-fold), and suppressed the pathogenic species such as Staphylococcaceae (0.03-fold) that, conversely, was enriched in the MJGT_H group (1.92-fold). Therefore, the biphasic effect indicated that the dosage of the herbal tea should not be overlooked.
摘要：
在中国山西省，毛坚茶（MJT）作为助消化剂已普遍食用了一百多年。然而,其疗效的确定仍然难以捉摸。本研究调查了毛尖绿茶（MJGT）对胃肠动力的影响。在体内鉴定了MJGT水提物对大鼠胃排空和小肠推进的双相作用；即，低（MJGT_L）和中（MJGT_M）浓度促进胃肠运动（p<0.05），而高浓度（MJGT_H）则表现出相反的作用（p<0.01）。胃激素的表达水平，GAS,MTL和VIP（p<0.05）与胃肠蠕动变化一致，MJGT_H组MTL除外(p>0.01)。两种黄酮类化合物，奥替多醇（0.152mg/mL）和木犀草素（0.034mg/mL），和相应的糖苷麦地醇-7-O-葡萄糖苷（0.637mg/mL）和木犀草素-7-O-葡萄糖苷（0.216mg/mL），通过HPLC和UPLC-ESI-MS鉴定的水提取物占主导地位。这些化合物可以调节从胃肠组织分离的肌条收缩。此外,不同的浓度也会影响以16SrDNA基因测序为特征的肠道微生物群。MJGT_L促进了几种益生菌，例如Muribaculaceae（1.77倍），Prevotellaceae（1.85倍）和乳杆菌（2.47倍），并抑制了致病物种，如葡萄球菌科（0.03倍），相反,在MJGT_H组中富集(1.92倍)。因此,双相效应表明凉茶的用量不容忽视。
