Abstract：
This study reports the synthesis of a nanocomposite consisting of spongin and its applicability in the development of an aptasensing platform with high performance. The spongin was carefully extracted from a marine sponge and decorated with copper tungsten oxide hydroxide. The resulting spongin-copper tungsten oxide hydroxide was functionalized by silver nanoparticles and utilized in electrochemical aptasensor fabrication. The nanocomposite covered on a glassy carbon electrode surface amplified the electron transfer and increased active electrochemical sites. The aptasensor was fabricated by loading of thiolated aptamer on the embedded surface via thiol-AgNPs linkage. The applicability of the aptasensor was tested in detecting the Staphylococcus aureus bacterium as one of the five most common causes of nosocomial infectious diseases. The aptasensor measured S. aureus under a linear concentration range of 10-108 colony-forming units per milliliter and a limit of quantification and detection of 12 and 1 colony-forming unit per milliliter, respectively. The highly selective diagnosis of S. aureus in the presence of some common bacterial strains was satisfactorily evaluated. The acceptable results of the human serum analysis as the real sample may be promising in the bacteria tracking in clinical samples underlying the green chemistry principle.
摘要：
这项研究报告了由海绵组成的纳米复合材料的合成及其在高性能适应平台开发中的适用性。从海洋海绵中小心提取海绵并用氢氧化铜钨装饰。所得海绵-氧化铜钨氢氧化物被银纳米颗粒官能化，并用于电化学传感器制造。覆盖在玻碳电极表面上的纳米复合材料放大了电子转移并增加了活性电化学位点。通过经由巯基-AgNP键将巯基化的适体负载在嵌入的表面上来制造适体。在检测金黄色葡萄球菌细菌作为医院感染性疾病的五个最常见原因之一时，测试了aptasensor的适用性。aptasensor在10-108菌落形成单位/毫升的线性浓度范围内测量金黄色葡萄球菌，定量和检测极限为12和1菌落形成单位/毫升，分别。满意地评估了在一些常见细菌菌株存在下金黄色葡萄球菌的高选择性诊断。作为真实样品的人血清分析的可接受结果在基于绿色化学原理的临床样品中的细菌追踪中可能是有希望的。
