Abstract：
Matrin 3 (MATR3) is one of the most abundant inner nuclear matrix proteins involved in multiple nuclear processes. However, to date, the biological role and prognostic relevance of MATR3 in human cancers still need to be explored. Therefore, the present study aimed to examine the expression levels and prognostic significance of MATR3 in clear cell renal cell carcinoma (ccRCC) patients. We assessed MATR3 immunohistochemical staining and RNA-seq data from publicly available data sets, and the results were analyzed with reference to clinicopathological characteristics and the overall survival of patients. Furthermore, the protein-protein interaction (PPI) network for MATR3 and its neighbors was constructed, functionally annotated, and screened for survival-related genes. MATR3 protein and mRNA levels were lower in tumor tissues compared to control tissues. Lower MATR3 protein (HR 2.36, 95%CI 1.41-3.97; p = 0.001) and mRNA (HR 2.01, 95%CI 1.46-2.75; p < 0.0001) expression levels were found to be a significant independent adverse prognostic factor for the patient\'s overall survival (OS). Moreover, of the candidate genes, the MRPL23 gene was identified as being the most predictive of OS, and combined MRPL23/MATR3 expression status predicted patient survival better than looking at each marker individually (HR 3.15, 95%CI 2.05-4.83; p < 0.0001). In conclusion, the results from the present investigation warrant further research into the biological and prognostic value of MATR3 and MRPL23 in ccRCC patients.
摘要：
Matrin3（MATR3）是参与多个核过程的最丰富的内核基质蛋白之一。然而,到目前为止,MATR3在人类癌症中的生物学作用和预后相关性仍需进一步探讨.因此,本研究旨在探讨MATR3在肾透明细胞癌（ccRCC）患者中的表达水平及其预后意义。我们评估了来自公开数据集的MATR3免疫组织化学染色和RNA-seq数据，并结合患者的临床病理特征和总体生存率对结果进行分析。此外，构建了MATR3及其邻居的蛋白质-蛋白质相互作用(PPI)网络，功能注释，并筛选与生存相关的基因。与对照组织相比,肿瘤组织中的MATR3蛋白和mRNA水平较低。较低的MATR3蛋白(HR2.36,95CI1.41-3.97;p=0.001)和mRNA(HR2.01,95CI1.46-2.75;p<0.0001)表达水平被发现是患者总生存期(OS)的显著独立不良预后因素。此外，候选基因，MRPL23基因被鉴定为对OS最具预测性的基因，联合MRPL23/MATR3表达状态预测患者生存率优于单独观察各标志物(HR3.15,95CI2.05-4.83;p<0.0001)。总之,本研究的结果需要进一步研究MATR3和MRPL23在ccRCC患者中的生物学和预后价值.
