PDF(Pubmed)
Abstract：
Antimicrobial resistance bacteria are nowadays ubiquitous. Its presence has been reported in almost every type of source, from water for agricultural and recreative use, water distribution pipes, and wastewater, to food, fomites, and clinical samples. Enterobacteriaceae, especially Escherichia coli, are not the exception, showing an increased resistance to several antibiotics, causing a global health and economic burden. Therefore, the monitoring of fecal microbiota is important because it is present in numerous reservoirs where gene transfer between commensal and virulent bacteria can take place, representing a potential source of resistant E. coli. In this work, antibiotic resistance profiles of 150 E. coli isolates from environmental, animal, and human samples, collected in three rural areas in Panama, were analyzed. A total of 116 isolates were resistant to at least one of the nine antibiotics tested. Remarkably, almost 100% of these exhibited resistance to tetracycline. Plasmid-associated tetA and tetB genes were detected in 42.86% of the isolates analyzed, tetA being the most prevalent. These results suggest that tetracycline resistance would be used as a convenient indicator of genetic horizontal transfer within a community.
摘要：
抗菌素耐药性细菌如今无处不在。几乎所有类型的来源都报告了它的存在，从农业和娱乐用水中，水分配管,和废水，食物，fomites,和临床样本。肠杆菌科,尤其是大肠杆菌,也不例外,显示出对几种抗生素的耐药性增加，造成全球健康和经济负担。因此,粪便微生物群的监测很重要，因为它存在于许多水库中，共生细菌和有毒细菌之间的基因转移可以发生，代表耐药大肠杆菌的潜在来源。在这项工作中,来自环境的150个大肠杆菌分离株的抗生素抗性概况，动物,和人体样本，收集在巴拿马的三个农村地区，进行了分析。总共116个分离株对测试的9种抗生素中的至少一种具有抗性。值得注意的是,其中几乎100％表现出对四环素的抗性。在分析的分离株中，有42.86%检测到质粒相关的tetA和tetB基因，tetA是最普遍的。这些结果表明，四环素抗性将被用作社区内遗传水平转移的方便指标。
