Abstract：
The calmodulin-like (CML) family is an important calcium (Ca2+) sensor in plants and plays a pivotal role in the response to abiotic and biotic stresses. As one of the most salt-tolerant grass species, Paspalums vaginatum is resistant to multiple abiotic stresses, such as salt, cold, and drought. However, investigations of PvCML proteins in P. vaginatum have been limited. Based on the recently published P. vaginatum genome, we identified forty-nine PvCMLs and performed a comprehensive bioinformatics analysis of PvCMLs. The main results showed that the PvCMLs were unevenly distributed on all chromosomes and that the expansion of PvCMLs was shaped by tandem and segmental duplications. In addition, cis-acting element analysis, expression profiles, and qRT-PCR analysis revealed that PvCMLs were involved in the response to salt and cold stress. Most interestingly, we found evidence of a tandem gene cluster that independently evolved in P. vaginatum and may participate in cold resistance. In summary, our work provides important insight into how grass species are resistant to abiotic stresses such as salt and cold and could be the basis of further gene function research on CMLs in P. vaginatum.
摘要：
钙调蛋白（CML）家族是植物中重要的钙（Ca2）传感器，在应对非生物和生物胁迫中起着关键作用。作为最耐盐的草种之一，阴道paspalums对多种非生物胁迫具有抗性，比如盐，冷,和干旱。然而,对阴道假单胞菌中PvCML蛋白的研究有限。根据最近发表的阴道假单胞菌基因组，我们确定了49个PvCML,并对PvCML进行了全面的生物信息学分析.主要结果表明，PvCML在所有染色体上的分布不均匀，PvCML的扩展是通过串联和分段重复形成的。此外,顺式作用元素分析，表达式配置文件,qRT-PCR分析表明，PvCML参与了对盐和冷胁迫的反应。最有趣的是，我们发现了一个串联基因簇的证据，该基因簇在阴道假单胞菌中独立进化并可能参与抗寒。总之,我们的工作提供了重要的见解，草物种如何抵抗非生物胁迫，如盐和寒冷，并可能是进一步的基因功能研究的基础。
