PDF(Pubmed)
Abstract：
Oxygen tension varies during placental and fetal development. Although hypoxia drives early trophoblast invasion, low placental oxygen levels during pregnancy show association with pregnancy complications including fetal growth restriction and preeclampsia. JEG-3 cells are often used as a trophoblast model. We studied transcriptional changes of JEG-3 cells on a uterine leiomyoma derived matrix Myogel. This might be the closest condition to the real uterine environment that we can get for an in vitro model. We observed that culturing JEG-3 cells on the leiomyoma matrix leads to strong stimulation of ribosomal pathways, energy metabolism, and ATP production. Furthermore, Myogel improved JEG-3 cell adherence in comparison to tissue culture treated plastic. We also included PDMS microchip hypoxia creation, and observed changes in oxidative phosphorylation, oxygen related genes and several hypoxia genes. Our study highlights the effects of Myogel matrix on growing JEG-3 cells, especially on mitochondria, energy metabolism, and protein synthesis.
摘要：
氧张力在胎盘和胎儿发育期间变化。虽然缺氧导致早期滋养层侵入，妊娠期胎盘氧水平低与妊娠并发症相关,包括胎儿生长受限和先兆子痫.JEG-3细胞通常用作滋养层模型。我们研究了JEG-3细胞在子宫平滑肌瘤衍生基质Myogel上的转录变化。这可能是我们可以获得的体外模型的最接近真实子宫环境的条件。我们观察到在平滑肌瘤基质上培养JEG-3细胞导致核糖体途径的强烈刺激，能量代谢，和ATP生产。此外，与组织培养处理的塑料相比,Myogel改善了JEG-3细胞粘附。我们还包括PDMS微芯片缺氧创建，并观察到氧化磷酸化的变化，氧相关基因和几个缺氧基因。我们的研究强调了Myogel基质对JEG-3细胞生长的影响，特别是在线粒体上，能量代谢，和蛋白质合成。
