Abstract：
Staphylococcus pseudintermedius is an opportunistic pathogen commonly associated with skin infections in dogs. Twenty-three methicillin-resistant S. pseudintermedius (MRSP) isolated in Argentina from dogs with pyoderma were analyzed using whole genome sequencing (WGS) and classified into sequence types (ST) by multilocus sequence typing (MLST) and staphylococcal chromosome cassette mec (SCCmec) types.Based on the WGS analysis, MLST, and SCCmec type results, we report for the first time in Argentina two MRSP strains, one each, belonging to ST71-SCCmec III and ST45-ΨSCCmec57395 from dogs with pyoderma. We also identified seven isolates with ST339, which had been previously reported in only two isolates in Argentina. Additionally, we identified ten MRSP isolates harboring variants of the SCCmec V found in S. aureus, seven SCCmec V (5C2&5) with two ccrC1 recombinases, and three SCCmec V (5C2) with one ccrC1 recombinase.Our findings provide important insights into the evolution and geographic spread of these hypervirulent dominant clones that threaten the health of our companion animals and represent a significant risk for zoonotic infections.
摘要：
假中间葡萄球菌是一种机会病原体，通常与狗的皮肤感染有关。使用全基因组测序（WGS）分析了在阿根廷从脓皮病犬中分离出的23种耐甲氧西林假中介杆菌（MRSP），并通过多位点序列分型（MLST）和葡萄球菌染色体盒mec（SCCmec）类型将其分为序列类型（ST）。基于WGS分析,MLST,和SCCmec类型的结果，我们首次在阿根廷报道了两种MRSP菌株，每个一个,来自脓皮病犬的属于ST71-SCCmecIII和ST45-kWSCCmec57395。我们还鉴定了7个ST339分离株，以前在阿根廷仅有2个分离株报道。此外,我们确定了十种MRSP分离株，这些分离株包含在金黄色葡萄球菌中发现的SCCmecV的变体，七个SCCmecV（5C2和5）与两个ccrC1重组酶，和三个具有一个ccrC1重组酶的SCCmecV(5C2)。我们的发现为这些高毒力优势克隆的进化和地理传播提供了重要的见解，这些克隆威胁着我们伴侣动物的健康，并代表了人畜共患感染的重大风险。
