PDF(Pubmed)
Abstract：
To identify novel solutions to improve rice yield under rising temperatures, molecular components of thermotolerance must be better understood. Alternative splicing (AS) is a major post-transcriptional mechanism impacting plant tolerance against stresses, including heat stress (HS). AS is largely regulated by splicing factors (SFs) and recent studies have shown their involvement in temperature response. However, little is known about the splicing networks between SFs and AS transcripts in the HS response. To expand this knowledge, we constructed a co-expression network based on a publicly available RNA-seq dataset that explored rice basal thermotolerance over a time-course. Our analyses suggest that the HS-dependent control of the abundance of specific transcripts coding for SFs might explain the widespread, coordinated, complex, and delicate AS regulation of critical genes during a plant\'s inherent response to extreme temperatures. AS changes in these critical genes might affect many aspects of plant biology, from organellar functions to cell death, providing relevant regulatory candidates for future functional studies of basal thermotolerance.
摘要：
为了找到在高温下提高水稻产量的新方法，必须更好地理解耐热性的分子成分。选择性剪接（AS）是影响植物对胁迫的耐受性的主要转录后机制，包括热应力（HS）。AS在很大程度上受到剪接因子（SF）的调节，最近的研究表明它们参与温度反应。然而,对HS应答中SF和AS转录物之间的拼接网络知之甚少。为了扩大这方面的知识，我们基于公开的RNA-seq数据集构建了一个共表达网络，该网络探索了水稻在一段时间内的基础耐热性。我们的分析表明，HS依赖性控制编码SF的特定转录本的丰度可能解释了广泛的，协调,复杂，以及植物对极端温度的固有反应过程中关键基因的微妙AS调节。这些关键基因的变化可能会影响植物生物学的许多方面，从细胞器功能到细胞死亡，为未来基础耐热性的功能研究提供相关的监管候选人。
