以 MAPKAPK2 为中心的转录组谱分析揭示了其在 HNSCC 发病过程中控制 IGFBP2 , MUC4 和 PRKAR2B 分子串扰的主要作用。
关键词: 3'-UTR 3′-UTR, 3′-untranslated region AREs, Adenylate-uridylate-rich element(s) ATCC, American Type Culture Collection ActD, Actinomycin D CISBP, Catalog of Inferred Sequence Binding Preferences Ct, Cycle Threshold DAP3, Death associated protein 3 DEGs, Differentially expressed gene(s) Differentially expressed genes EHBP1, EH domain binding protein 1 FC, Fold change FDR, False discovery rate FPKM, Fragments per kilobase of transcript per million mapped GFP, Green fluorescent protein GO, Gene Ontology HKG, House-keeping genes HNSCC HNSCCs, Head and neck squamous cell carcinoma(s) HQ, High quality IAEC, Institutional animal ethics committee IFN, Interferon IGFBP2, Insulin-like growth factor-binding protein 2 IHC, Immunohistochemistry IP6K2, Inositol hexakisphosphate kinase 2 KD, Knockdown KEGG, Kyoto encyclopedia of genes and genomics MAPK, Mitogen-Activated Protein Kinase MAPKAPK2 MAPKAPK2 or MK2, Mitogen-activated protein kinase-activated protein kinase 2 MELK, Maternal embryonic leucine zipper kinase MK2KD, MK2-knockdown MK2WT, MK2 wild-type MKP-1, Mitogen-activated protein kinase phosphatase-1 MUC4, Mucin 4 NGS, Next generation sequencing NOD/SCID, Non-obese diabetic/severe combined immunodeficient PRKAR2B, Protein kinase CAMP-dependent type II regulatory subunit beta QC, Quality control RBPs, RNA-binding protein(s) RIN, RNA integrity number RNA-seq, Ribose Nucleic Acid -sequencing RNA-sequencing RT-qPCR, Real-time quantitative polymerase chain reaction RUNX1, Runt-related transcription factor 1 SLF2, SMC5-SMC6 complex localization factor 2 TCGA, The cancer genome atlas TNF-α, Tumor necrosis factor-alpha TTP, Tristetraprolin Transcriptome VEGF, Vascular endothelial growth factor WB, Western blotting WT, Wild type ZNF662, Zinc finger protein 662 p27, Cyclin-dependent kinase inhibitor 1B shRNA, Short hairpin RNA
来 源:
DOI:10.1016/j.csbj.2023.01.039
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