PDF(Pubmed)
Abstract：
Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments, such as high-glucose, excessive reactive oxygen species (ROS), hypoxia and malnutrition. Unfortunately, few strategies have been developed to ameliorate the multiple microenvironments simultaneously. In this study, Chlorella sp. (Chlorella) hydrogels were prepared against diabetic wounds. In vitro experiments demonstrated that living Chlorella could produce dissolved oxygen by photosynthesis, actively consume glucose and deplete ROS with the inherent antioxidants, during the daytime. At night, Chlorella was inactivated in situ by chlorine dioxide with human-body harmless concentration to utilize its abundant contents. It was verified in vitro that the inactivated-Chlorella could supply nutrition, relieve inflammation and terminate the oxygen-consumption of Chlorella-respiration. The advantages of living Chlorella and its contents were integrated ingeniously. The abovementioned functions were proven to accelerate cell proliferation, migration and angiogenesis in vitro. Then, streptozotocin-induced diabetic mice were employed for further validation. The in vivo outcomes confirmed that Chlorella could ameliorate the undesirable microenvironments, including hypoxia, high-glucose, excessive-ROS and chronic inflammation, thereby synergistically promoting tissue regeneration. Given the results above, Chlorella is considered as a tailor-made therapeutic strategy for diabetic wound healing.
摘要：
慢性糖尿病伤口仍然是一个严峻的挑战，来自复杂的负面微环境，比如高葡萄糖，过量的活性氧（ROS），缺氧和营养不良。不幸的是,很少有策略同时改善多个微环境。在这项研究中,小球藻sp.针对糖尿病伤口制备(小球藻)水凝胶。体外实验表明，活小球藻可以通过光合作用产生溶解氧，积极消耗葡萄糖和消耗ROS与固有的抗氧化剂，在白天。晚上,小球藻被人体无害浓度的二氧化氯原位灭活，以利用其丰富的含量。体外验证了灭活小球藻可以提供营养，减轻炎症，终止小球藻呼吸的耗氧量。巧妙地整合了生活小球藻的优点及其内容。上述功能被证明可以加速细胞增殖，体外迁移和血管生成。然后，使用链脲佐菌素诱导的糖尿病小鼠进行进一步验证。体内结果证实小球藻可以改善不良的微环境，包括缺氧,高葡萄糖，过量的ROS和慢性炎症，从而协同促进组织再生。鉴于上述结果，小球藻被认为是糖尿病伤口愈合的量身定制的治疗策略。
