PDF(Pubmed)
Abstract：
Meat quality traits (MQTs) have gained more attention from breeders due to their increasing economic value in the commercial pig industry. In this genome-wide association study (GWAS), 223 four-way intercross pigs were genotyped using the specific-locus amplified fragment sequencing (SLAF-seq) and phenotyped for PH at 45 min post mortem (PH45), meat color score (MC), marbling score (MA), water loss rate (WL), drip loss (DL) in the longissimus muscle, and cooking loss (CL) in the psoas major muscle. A total of 227, 921 filtered single nucleotide polymorphisms (SNPs) evenly distributed across the entire genome were detected to perform GWAS. A total of 64 SNPs were identified for six meat quality traits using the mixed linear model (MLM), of which 24 SNPs were located in previously reported QTL regions. The phenotypic variation explained (PVE) by the significant SNPs was from 2.43% to 16.32%. The genomic heritability estimates based on SNP for six meat-quality traits were low to moderate (0.07-0.47) being the lowest for CL and the highest for DL. A total of 30 genes located within 10 kb upstream or downstream of these significant SNPs were found. Furthermore, several candidate genes for MQTs were detected, including pH45 (GRM8), MC (ANKRD6), MA (MACROD2 and ABCG1), WL (TMEM50A), CL (PIP4K2A) and DL (CDYL2, CHL1, ABCA4, ZAG and SLC1A2). This study provided substantial new evidence for several candidate genes to participate in different pork quality traits. The identification of these SNPs and candidate genes provided a basis for molecular marker-assisted breeding and improvement of pork quality traits.
摘要：
肉质性状（MQT）由于其在商业养猪业中不断增加的经济价值而受到育种者的更多关注。在这项全基因组关联研究(GWAS)中，使用特定基因座扩增片段测序（SLAF-seq）对223头四向杂交猪进行基因分型，并在死后45分钟（PH45）对PH进行表型分型，肉类颜色评分（MC），大理石花纹得分（MA），失水率（WL），长肌的滴水损失（DL），和腰大肌的烹饪损失（CL）。检测到在整个基因组中均匀分布的总共227,921个过滤的单核苷酸多态性(SNP)以进行GWAS。使用混合线性模型（MLM）鉴定了六个肉质性状的64个SNP，其中24个SNP位于先前报道的QTL区域。显著SNPs解释的表型变异(PVE)为2.43%~16.32%。基于SNP的六个肉质性状的基因组遗传力估计为低至中等（0.07-0.47），CL最低，DL最高。发现了位于这些重要SNP上游或下游10kb内的总共30个基因。此外，检测到几个MQT的候选基因，包括pH45(GRM8)，MC(ANKRD6),MA（MACROD2和ABCG1），WL(TMEM50A),CL(PIP4K2A)和DL(CDYL2、CHL1、ABCA4、ZAG和SLC1A2)。本研究为几个候选基因参与不同猪肉品质性状提供了大量新的证据。这些SNP和候选基因的鉴定为分子标记辅助育种和猪肉品质性状的改良提供了依据。
