OBJECTIVE: The objective of this study was to determine the antimicrobial activity of the hexanic extract (HE) of Achyrocline satureioides on P. larvae and the inhibitory activity on some mechanisms related to pathogenicity.
METHODS: The Minimum Inhibitory Concentration (MIC) of the HE was determined by the broth microdilution technique and the Minimum Bactericidal Concentration (MBC) by the microdrop technique. Swimming and swarming motility was evaluated in plates with 0.3 and 0.5% agar, respectively. Biofilm formation was evaluated and quantified by the Congo red and crystal violet method. The protease activity was evaluated by the qualitative technique on skim milk agar plates.
RESULTS: It was determined that the MIC of the HE on four strains of P. larvae ranged between 0.3 and 9.37 µg/ml and the MBC between 1.17 and 150 µg/ml. On the other hand, sub-inhibitory concentrations of the HE were able to decrease swimming motility, biofilm formation and the proteases production of P. larvae.
目的:本研究的目的是确定阿奇罗兰的六氢提取物(HE)对P.幼虫的抗菌活性及其对某些致病机制的抑制活性。
方法:通过肉汤微量稀释技术测定HE的最低抑菌浓度(MIC),通过微滴技术测定最低杀菌浓度(MBC)。
结果:确定HE对4种P.larvae的MIC在0.3至9.37µg/ml之间,MBC在1.17至150µg/ml之间。