Abstract：
We investigated the impacts of photobiomodulation (PBM) and human allogeneic adipose-derived stem cells (ha-ADS) together and or alone applications on the stereological parameters, immunohistochemical characterizing of M1 and M2 macrophages, and mRNA levels of hypoxia-inducible factor (HIF-1α), basic fibroblast growth factor (bFGF), vascular endothelial growth factor-A (VEGF-A) and stromal cell-derived factor-1α (SDF-1α) on inflammation (day 4) and proliferation phases (day 8) of repairing tissues in an infected delayed healing and ischemic wound model (IDHIWM) in type 1 diabetic (DM1) rats. DM1 was created in 48 rats and an IDHIWM was made in all of them, and they were distributed into 4 groups. Group1 = control rats with no treatment. Group2 = rats received (10 × 100000 ha-ADS). Group3 = rats exposed to PBM (890 nm, 80 Hz, 3.46 J/cm2). Group4 = rats received both PBM and ha-ADS. On day 8, there were significantly higher neutrophils in the control group than in other groups (p < 0.01). There were substantially higher macrophages in the PBM + ha-ADS group than in other groups on days 4 and 8 (p < 0.001). Granulation tissue volume, on both days 4 and 8, was meaningfully greater in all treatment groups than in the control group (all, p = 0.000). Results of M1 and M2 macrophage counts of repairing tissue in the entire treatment groups were considered preferable to those in the control group (p < 0.05). Regarding stereological and macrophage phenotyping, the results of the PBM + ha-ADS group were better than the ha-ADS and PBM groups. Results of the tested gene expression of repairing tissue on inflammation and proliferation steps in PBM and PBM + ha-ADS groups were meaningfully better than the control and ha-ADS groups (p < 0.05). We showed that PBM, ha-ADS, and PBM plus ha-ADS, hastened the proliferation step of healing in an IDHIWM in rats with DM1 by regulation of the inflammatory reaction, macrophage phenotyping, and augmented granulation tissue formation. In addition PBM and PBM plus ha-ADS protocols hastened and increased mRNA levels of HIF-1α, bFGF, SDF-1α, and VEGF-A. Totally, in terms of stereological and immuno-histological tests, and also gene expression HIF-1α and VEGF-A, the results of PBM + ha-ADS were superior (additive) to PBM, and ha-ADS alone treatments.
摘要：
我们研究了光生物调节（PBM）和人类同种异体脂肪干细胞（ha-ADS）一起或单独应用对立体参数的影响，M1和M2巨噬细胞的免疫组织化学特征，缺氧诱导因子（HIF-1α）的mRNA水平，碱性成纤维细胞生长因子（bFGF），血管内皮生长因子-A（VEGF-A）和基质细胞衍生因子-1α（SDF-1α）在1型糖尿病（DM1）大鼠感染延迟愈合和缺血伤口模型（IDHIWM）中修复组织的炎症（第4天）和增殖期（第8天）。在48只大鼠中产生了DM1，并在所有大鼠中制造了IDHIWM，他们被分成4组。组1=未处理的对照大鼠。组2=接受的大鼠(10×100000ha-ADS)。Group3=暴露于PBM的大鼠（890nm，80Hz,3.46J/cm2)。组4=大鼠接受PBM和ha-ADS。在第8天，对照组的中性粒细胞明显高于其他组（p<0.01）。在第4天和第8天,PBM+ha-ADS组中的巨噬细胞明显高于其他组(p<0.001)。肉芽组织体积,在第4天和第8天，所有治疗组均明显高于对照组（所有，p=0.000）。整个治疗组中修复组织的M1和M2巨噬细胞计数的结果被认为优于对照组(p<0.05)。关于体视学和巨噬细胞表型，PBM+ha-ADS组的结果优于ha-ADS和PBM组。PBM和PBMha-ADS组的炎症和增殖步骤的修复组织基因表达检测结果明显优于对照组和ha-ADS组（p<0.05）。我们发现PBM,HA-ADS,PBM加HA-ADS,通过调节炎症反应加快了DM1大鼠IDHIWM愈合的增殖步骤，巨噬细胞表型，和增加肉芽组织的形成。此外，PBM和PBM加ha-ADS方案加快并增加了HIF-1α的mRNA水平，bFGF,SDF-1α,和VEGF-A。完全正确,在体视学和免疫组织学测试方面，以及HIF-1α和VEGF-A的基因表达，PBM+HA-ADS的结果(添加剂)优于PBM，和HA-ADS单独治疗。
