PDF(Pubmed)
Abstract：
Eicosapolyenoic fatty acids are integral components of oomycete pathogens that can act as microbe-associated molecular patterns to induce disease resistance in plants. Defense-inducing eicosapolyenoic fatty acids include arachidonic acid (AA) and eicosapentaenoic acid and are strong elicitors in solanaceous plants, with bioactivity in other plant families. Similarly, extracts of a brown seaweed, Ascophyllum nodosum, used in sustainable agriculture as a biostimulant of plant growth, may also induce disease resistance. A. nodosum, similar to other macroalgae, is rich in eicosapolyenoic fatty acids, which comprise as much as 25% of total fatty acid composition. We investigated the response of roots and leaves from AA or a commercial A. nodosum extract (ANE) on root-treated tomatoes via RNA sequencing, phytohormone profiling, and disease assays. AA and ANE significantly altered transcriptional profiles relative to control plants, inducing numerous defense-related genes with both substantial overlap and differences in gene expression patterns. Root treatment with AA and, to a lesser extent, ANE also altered both salicylic acid and jasmonic acid levels while inducing local and systemic resistance to oomycete and bacterial pathogen challenge. Thus, our study highlights overlap in both local and systemic defense induced by AA and ANE, with potential for inducing broad-spectrum resistance against pathogens. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
摘要：
二十碳烯脂肪酸是卵菌病原体的组成部分，可以作为微生物相关分子模式（MAMPs）诱导植物抗病性。诱导防御的二十碳烯脂肪酸包括花生四烯酸（AA）和二十碳五烯酸，并且是茄科植物中的强激发子，在其他植物科中具有生物活性。同样，棕色海藻的提取物，夜蛾，在可持续农业中用作植物生长的生物刺激剂，也可能引起抗病性。A.nodosum,与其他大型藻类相似，富含二十碳烯脂肪酸，占总脂肪酸组成的25%。我们通过RNA测序研究了AA或商业A.nodosum提取物（ANE）对根处理过的番茄的根和叶的反应，植物激素分析，和疾病检测。AA和ANE相对于对照植物显著改变转录谱，诱导许多与防御相关的基因，这些基因既有实质性的重叠，也有基因表达模式的差异。根处理与AA和，在较小程度上，ANE还改变了水杨酸和茉莉酸的水平，同时诱导了对卵菌和细菌病原体攻击的局部和系统抗性。因此，我们的研究强调了AA和ANE引起的局部和系统防御的重叠，具有诱导针对病原体的广谱抗性的潜力。
