PDF(Pubmed)
Abstract：
Numerous fungicide applications are required to control Erysiphe necator, the causative agent of powdery mildew. This increased demand for cultivars with strong and long-lasting field resistance to diseases and pests. In comparison to the susceptible cultivar \'Teroldego\', the current study provides information on some promising disease-resistant varieties (mono-locus) carrying one E. necator-resistant locus: BC4 and \'Kishmish vatkana\', as well as resistant genotypes carrying several E. necator resistant loci (pyramided): \'Bianca\', F26P92, F13P71, and NY42. A clear picture of the metabolites\' alterations in response to the pathogen is shown by profiling the main and secondary metabolism: primary compounds and lipids; volatile organic compounds and phenolic compounds at 0, 12, and 48 hours after pathogen inoculation. We identified several compounds whose metabolic modulation indicated that resistant plants initiate defense upon pathogen inoculation, which, while similar to the susceptible genotype in some cases, did not imply that the plants were not resistant, but rather that their resistance was modulated at different percentages of metabolite accumulation and with different effect sizes. As a result, we discovered ten up-accumulated metabolites that distinguished resistant from susceptible varieties in response to powdery mildew inoculation, three of which have already been proposed as resistance biomarkers due to their role in activating the plant defense response.
摘要：
需要许多杀菌剂应用来控制Erysiphenecator，白粉病的病原体。这增加了对具有对病虫害的强大和持久的田间抗性的品种的需求。与易感品种“Teroldego”相比，当前的研究提供了一些有希望的抗病品种（单基因座）携带一个E.necaator抗性基因座的信息：BC4和“Kishmishvatkana”，以及携带几个E.necator抗性基因座的抗性基因型（金字塔）：\'Bianca\'，F26P92、F13P71和NY42。通过分析主要和次要代谢：主要化合物和脂质；病原体接种后0、12和48小时的挥发性有机化合物和酚类化合物，可以清楚地显示出响应病原体的代谢物变化。我们确定了几种化合物，其代谢调节表明抗性植物在病原体接种后启动防御，which,虽然在某些情况下与易感基因型相似，并不意味着植物没有抗性，而是它们的抗性在不同的代谢物积累百分比和不同的效应大小下被调节。因此,我们发现了十种向上积累的代谢产物，它们在白粉病接种时将抗性与易感品种区分开来，由于它们在激活植物防御反应中的作用，其中三种已经被提出作为抗性生物标志物。
