PDF(Pubmed)
Abstract：
Phytohormones represent a group of secondary metabolites with different chemical structures, in which belong auxins, cytokinins, gibberellins, or brassinosteroids. In higher plants, they cover active roles in growth or defense function, while their potential benefits for human health protection were noted for some phytohormones and little explored for many others. In this study, we developed a target fishing strategy on fifty-three selected naturally occurring phytohormones covering different families towards proteins involved in key cellular functions related to human metabolism and health protection/disease. This in silico analysis strategy aims to screen the potential human health-driven bioactivity of more than fifty phytohormones through the analysis of their interactions with specific targets. From this analysis, twenty-eight human targets were recovered. Some targets e.g., the proteins mitochondrial glutamate dehydrogenase (GLUD1) or nerve growth factor (NGF) bound many phytohormones, highlighting their involvement in amino acid metabolism and/or in the maintenance or survival of neurons. Conversely, some phytohormones specifically interacted with some proteins, e.g., SPRY domain-containing SOCS box protein 2 (SPSB2) or Inosine-5\'-monophosphate dehydrogenase 1 (IMPDH1), both involved in human immune response. They were then investigated with a molecular docking analysis approach. Our bioprospecting study indicated that many phytohormones may endow human health benefits, with potential functional role in multiple cellular processes including immune response and cell cycle progression.
摘要：
植物激素代表一组具有不同化学结构的次级代谢产物，其中属于生长素，细胞分裂素,赤霉素,或者油菜素类固醇.在高等植物中,它们涵盖了生长或防御功能的积极作用，虽然它们对人类健康保护的潜在益处在一些植物激素中被注意到，但在许多其他激素中很少被探索。在这项研究中,我们制定了针对53种选定的天然植物激素的目标捕捞策略，涵盖了涉及与人类代谢和健康保护/疾病相关的关键细胞功能的蛋白质的不同家族。这种计算机分析策略旨在通过分析其与特定靶标的相互作用来筛选超过50种植物激素的潜在人类健康驱动的生物活性。从这个分析,二十八个人类目标被发现。一些目标，例如，蛋白质线粒体谷氨酸脱氢酶（GLUD1）或神经生长因子（NGF）结合许多植物激素，强调它们参与氨基酸代谢和/或神经元的维持或存活。相反，一些植物激素特别与一些蛋白质相互作用，例如，含SPRY结构域的SOCS盒蛋白2（SPSB2）或肌苷-5'-单磷酸脱氢酶1（IMPDH1），两者都参与人体免疫反应。然后用分子对接分析方法研究它们。我们的生物勘探研究表明，许多植物激素可能赋予人类健康益处，在包括免疫反应和细胞周期进程在内的多种细胞过程中具有潜在的功能作用。
