Abstract：
The emerging pathogen Campylobacter concisus has been isolated from patients with gastrointestinal diseases; however, it is also present in the gut of healthy individuals. The aim of this study was to compare IL-8 production in HT-29 cells after infection with C. concisus from different gastrointestinal disease phenotypes. Additionally, to investigate whether differentiation of isolates in genomospecies (GS1 and GS2) or presence of the zot gene, encoding the Zot toxin, affects IL-8 production. A total of 37 C. concisus isolates from patients with microscopic colitis (n = 20), ulcerative colitis (n = 5), Crohn\'s disease (n = 5), diarrhoea (n = 2) and from healthy controls (n = 5) were used. Intestinal HT-29 cells were infected and incubated for 24 h. Supernatants were subsequently removed and analysed for IL-8 by MILLIPLEX. All isolates were able to stimulate IL-8 production and IL-8 levels were higher than in non-infected HT-29 cells. No difference was observed between disease phenotypes or GS1 and GS2, whereas presence of the zot gene showed a tendency towards higher IL-8 production. Further investigations in other inflammatory and physiological models are needed to conclude whether C. concisus strains from different gastrointestinal disease phenotypes differ in pathogenic potential and play a part in gastrointestinal disease.
摘要：
已从患有胃肠道疾病的患者中分离出了新出现的病原体弯曲杆菌；然而，它也存在于健康个体的肠道中。这项研究的目的是比较感染来自不同胃肠道疾病表型的C.concisus后HT-29细胞中IL-8的产生。此外,为了研究基因组（GS1和GS2）中分离株的分化或zot基因的存在，编码Zot毒素,影响IL-8的产生。从显微镜下结肠炎患者（n=20）中分离出37株。溃疡性结肠炎（n=5），克罗恩病（n=5），使用腹泻（n=2）和健康对照（n=5）。感染肠HT-29细胞并孵育24小时。随后除去上清液并通过MILLIPLEX分析IL-8。所有分离物均能够刺激IL-8产生,并且IL-8水平高于未感染的HT-29细胞。在疾病表型或GS1和GS2之间没有观察到差异，而zot基因的存在显示出更高的IL-8产生趋势。需要在其他炎症和生理模型中进行进一步研究，以得出结论，来自不同胃肠道疾病表型的C.concisus菌株是否在致病潜力上有所不同并在胃肠道疾病中起作用。
