PDF(Pubmed)
Abstract：
UNASSIGNED: Brazil is a country endemic for human T-lymphotropic virus 1 and 2 (HTLV-1 and HTLV-2), systemic mycoses such as paracoccidioidomycosis (PCM) and histoplasmosis (HP), and aspergillosis (AP). The prevalence of HTLV-1/-2 infections in individuals with endemic mycoses in Latin America is unknown; however, an association between HTLV-1 and severe PCM and HP has been observed in Peru. Addressing this knowledge gap, we searched for HTLV-1/-2 antibodies in serum samples sent to the Instituto Adolfo Lutz, São Paulo, Brazil, for systemic mycosis diagnosis.
UNASSIGNED: We used 387 sera from a biorepository that had seropositive results for Paracoccidioides spp. (G1, n=212), Histoplasma capsulatum (G2, n=95), Aspergillus spp. (G3, n=61), and at least two of these fungi (G4, n=19). We searched for the presence of HTLV-1/-2 antibodies using commercial immunoassays: enzyme immunoassay (HTLV-I+II Murex, Diasorin), western blotting (HTLV Blot 2.4, MP Biomedicals), and line immunoassay (INNO-LIA HTLV I/II, Fujirebio). Demographic characteristics were evaluated in each group.
UNASSIGNED: Different regions in São Paulo were sampled. Most samples were from males (76.2%; p=0.001), except for G3, in which no sex bias was detected. Mean age differences were observed between groups: patients with PCM and HP had a similar mean age (42.8 and 42.0 years, respectively), while those with AP and co-fungal infection were older (55.1 and 52.8 years, respectively, (p<0.001). Noteworthy, males were older than females in G1 (p=0.005). Screening detected HTLV-1/2 antibodies in five samples (1.30%; 95% CI: 0.8-1.8%), with two borderline results. HTLV-1/2 was confirmed in two samples: 2/387 (0.52%; 0.063-1.85%): one HTLV-2, male, 42 years, from G1: 1/212 (0.47%; 0.012-2.60%), and one HTLV-1, male, 51 years, from G3: 1/61 (1.64%; 0.042-8.80%).
UNASSIGNED: In the state of São Paulo, HTLV-1 and HTLV-2 seem to circulate in male patients with systemic mycoses, and since HTLV-1 could impact fungal disease severity, the identification of co-infection is important regardless of prevalence.
UNASSIGNED: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), and Instituto Adolfo Lutz.
UNASSIGNED: We used 387 sera from a biorepository that had seropositive results for Paracoccidioides spp. (G1, n=212), Histoplasma capsulatum (G2, n=95), Aspergillus spp. (G3, n=61), and at least two of these fungi (G4, n=19). We searched for the presence of HTLV-1/-2 antibodies using commercial immunoassays: enzyme immunoassay (HTLV-I+II Murex, Diasorin), western blotting (HTLV Blot 2.4, MP Biomedicals), and line immunoassay (INNO-LIA HTLV I/II, Fujirebio). Demographic characteristics were evaluated in each group.
UNASSIGNED: Different regions in São Paulo were sampled. Most samples were from males (76.2%; p=0.001), except for G3, in which no sex bias was detected. Mean age differences were observed between groups: patients with PCM and HP had a similar mean age (42.8 and 42.0 years, respectively), while those with AP and co-fungal infection were older (55.1 and 52.8 years, respectively, (p<0.001). Noteworthy, males were older than females in G1 (p=0.005). Screening detected HTLV-1/2 antibodies in five samples (1.30%; 95% CI: 0.8-1.8%), with two borderline results. HTLV-1/2 was confirmed in two samples: 2/387 (0.52%; 0.063-1.85%): one HTLV-2, male, 42 years, from G1: 1/212 (0.47%; 0.012-2.60%), and one HTLV-1, male, 51 years, from G3: 1/61 (1.64%; 0.042-8.80%).
UNASSIGNED: In the state of São Paulo, HTLV-1 and HTLV-2 seem to circulate in male patients with systemic mycoses, and since HTLV-1 could impact fungal disease severity, the identification of co-infection is important regardless of prevalence.
UNASSIGNED: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), and Instituto Adolfo Lutz.
摘要：
未经评估：巴西是人类嗜T淋巴细胞病毒1和2（HTLV-1和HTLV-2）的流行国家，全身性真菌病，如副角菌病（PCM）和组织胞浆菌病（HP），和曲霉病（AP）。在拉丁美洲有地方性真菌病的个体中HTLV-1/-2感染的患病率是未知的;然而,在秘鲁观察到HTLV-1与重度PCM和HP之间的关联。解决这一知识差距，我们在送往AdolfoLutz研究所的血清样本中搜索了HTLV-1/-2抗体，圣保罗,巴西,用于全身真菌病诊断。
UNASSIGNED：我们使用了来自生物储存库的387份血清，该血清对副球菌属具有血清阳性结果。（G1，n=212），组织胞浆（G2，n=95），曲霉属。（G3，n=61），和这些真菌中的至少两种（G4，n=19）。我们使用商业免疫测定法搜索HTLV-1/-2抗体的存在：酶免疫测定法（HTLV-IIIMurex，Diasorin),蛋白质印迹（HTLV印迹2.4，MP生物医学），和线免疫测定(INNO-LIAHTLVI/II，Fujirebio）。评估每组的人口统计学特征。
UNASSIGNED：对圣保罗的不同地区进行了采样。大多数样本来自男性（76.2％；p=0.001），除了G3，没有检测到性别偏见。观察到两组之间的平均年龄差异：PCM和HP患者的平均年龄相似（42.8和42.0岁，分别)，而患有AP和共真菌感染的人年龄较大(55.1和52.8岁，分别,（p<0.001）。值得注意的是,G1期男性年龄大于女性（p=0.005）。筛选检测到5个样本中的HTLV-1/2抗体(1.30%;95%CI:0.8-1.8%),有两个临界结果。HTLV-1/2在两个样本中得到证实：2/387（0.52％;0.063-1.85％）：一个HTLV-2，男性，42年,从G1：1/212（0.47%；0.012-2.60%），还有一个HTLV-1男性,51年,从G3：1/61（1.64%；0.042-8.80%）。
未经评估：在圣保罗州，HTLV-1和HTLV-2似乎在患有全身性真菌病的男性患者中循环，由于HTLV-1会影响真菌病的严重程度，无论患病率如何,共同感染的识别都很重要.
UNASIGNED：CoordenaçãdeAperfeiçoamentodePessoaldeNívelSuperior（CAPES），圣保罗议会基金会（FAPESP），阿道夫·卢茨研究所。
UNASSIGNED：我们使用了来自生物储存库的387份血清，该血清对副球菌属具有血清阳性结果。
UNASSIGNED：对圣保罗的不同地区进行了采样。
未经评估：在圣保罗州，
UNASIGNED：CoordenaçãdeAperfeiçoamentodePessoaldeNívelSuperior（CAPES），
