PDF(Pubmed)
Abstract：
UNASSIGNED: Thioredoxin-1 (TXN), a redox balance factor, plays an essential role in oxidative stress and has been shown to act as a potential contributor to various cancers. This study evaluated the role of TXN in lung cancer by bioinformatics analyses.
UNASSIGNED: Genes differentially expressed in lung cancer and oxidative stress related genes were obtained from The Cancer Genome Atlas, Gene Expression Omnibus and GeneCards databases. Following identification of TXN as an optimal differentially expressed gene by bioinformatics, the prognostic value of TXN in lung cancer was evaluated by univariate/multivariate Cox regression and Kaplan-Meier survival analyses, with validation by receiver operation characteristic curve analysis. The association between TXN expression and lung cancer was verified by immunohistochemical analysis of the Human Protein Atlas database, as well as by western blotting and qPCR. Cell proliferation was determined by cell counting kit-8 after changing TXN expression using lentiviral transfection.
UNASSIGNED: Twenty differentially expressed oxidative stress genes were identified. Differential expression analysis identified five genes (CASP3, CAT, TXN, GSR, and HSPA4) and Kaplan-Meier survival analysis identified four genes (IL-6, CYCS, TXN, and BCL2) that differed significantly in lung cancer and normal lung tissue, indicating that TXN was an optimal differentially expressed gene. Multivariate Cox regression analysis showed that T stage (T3/T4), N stage (N2/N3), curative effect (progressive diseases) and high TXN expression were associated with poor survival, although high TXN expression was poorly predictive of overall survival. TXN was highly expressed in lung cancer tissues and cells. Knockdown of TXN suppressed cell proliferation, while overexpression of TXN enhanced cell proliferation.
UNASSIGNED: High expression of TXN plays an important role in lung cancer development and prognosis. Because it is a prospective prognostic factor, targeting TXN may have clinical benefits in the treatment of lung cancer.
UNASSIGNED: Genes differentially expressed in lung cancer and oxidative stress related genes were obtained from The Cancer Genome Atlas, Gene Expression Omnibus and GeneCards databases. Following identification of TXN as an optimal differentially expressed gene by bioinformatics, the prognostic value of TXN in lung cancer was evaluated by univariate/multivariate Cox regression and Kaplan-Meier survival analyses, with validation by receiver operation characteristic curve analysis. The association between TXN expression and lung cancer was verified by immunohistochemical analysis of the Human Protein Atlas database, as well as by western blotting and qPCR. Cell proliferation was determined by cell counting kit-8 after changing TXN expression using lentiviral transfection.
UNASSIGNED: Twenty differentially expressed oxidative stress genes were identified. Differential expression analysis identified five genes (CASP3, CAT, TXN, GSR, and HSPA4) and Kaplan-Meier survival analysis identified four genes (IL-6, CYCS, TXN, and BCL2) that differed significantly in lung cancer and normal lung tissue, indicating that TXN was an optimal differentially expressed gene. Multivariate Cox regression analysis showed that T stage (T3/T4), N stage (N2/N3), curative effect (progressive diseases) and high TXN expression were associated with poor survival, although high TXN expression was poorly predictive of overall survival. TXN was highly expressed in lung cancer tissues and cells. Knockdown of TXN suppressed cell proliferation, while overexpression of TXN enhanced cell proliferation.
UNASSIGNED: High expression of TXN plays an important role in lung cancer development and prognosis. Because it is a prospective prognostic factor, targeting TXN may have clinical benefits in the treatment of lung cancer.
摘要：
未经授权：硫氧还蛋白-1（TXN），氧化还原平衡因子，在氧化应激中起着至关重要的作用，并已被证明是各种癌症的潜在贡献者。本研究通过生物信息学分析评估TXN在肺癌中的作用。
UNASSIGNED：肺癌中差异表达的基因和氧化应激相关基因来自癌症基因组图谱，基因表达Omnibus和GeneCards数据库。在通过生物信息学鉴定TXN为最佳差异表达基因后，通过单因素/多因素Cox回归和Kaplan-Meier生存分析评估TXN在肺癌中的预后价值,通过接收器操作特性曲线分析进行验证。TXN表达与肺癌之间的关联通过人蛋白图谱数据库的免疫组织化学分析来验证。以及通过蛋白质印迹和qPCR。在使用慢病毒转染改变TXN表达后,通过细胞计数试剂盒-8测定细胞增殖。
未经鉴定：鉴定了20个差异表达的氧化应激基因。差异表达分析确定了五个基因(CASP3，CAT，TXN,GSR,和HSPA4）和Kaplan-Meier生存分析确定了四个基因（IL-6，CYCS，TXN,和BCL2）在肺癌和正常肺组织中存在显着差异，表明TXN是最佳的差异表达基因。多因素Cox回归分析显示T分期(T3/T4),N级(N2/N3)；疗效（进行性疾病）和高TXN表达与低生存率相关，尽管高TXN表达对总体生存率的预测能力较差。TXN在肺癌组织和细胞中高表达。敲除TXN抑制细胞增殖，而TXN的过表达增强了细胞增殖。
UNASSIGNED：TXN的高表达在肺癌的发展和预后中起重要作用。因为它是一个前瞻性的预后因素，靶向TXN在肺癌治疗中可能具有临床益处.
UNASSIGNED：肺癌中差异表达的基因和氧化应激相关基因来自癌症基因组图谱，
未经鉴定：鉴定了20个差异表达的氧化应激基因。
UNASSIGNED：TXN的高表达在肺癌的发展和预后中起重要作用。
