Abstract：
Quantitation of microRNAs secreted by lung cells can provide valuable information regarding lung health. Exhaled breath condensate (EBC) offers a non-invasive way to sample the secreted microRNAs, and could be used as diagnostic tools for lung cancer.
EBC samples from twenty treatment-naïve patients with pathologically confirmed lung cancer and twenty healthy subjects were profiled for miRNAs expression. Selected microRNAs were further validated, using quantitative-PCR, in an independent set of 10 subjects from both groups.
A total of 78 miRNAs were found to be significantly upregulated in the EBC of lung cancer patients compared to the control group. Six of these 78 miRNAs were shortlisted for validation. Of these, miR-31-3p, let7i, and miR-449c were significantly upregulated, exhibited good discriminatory power.
Differential expression of miRNAs secreted by lung cells could be quantitated in EBC samples, and could be used as a potential non-invasive tool for early diagnosis of lung cancer.
EBC samples from twenty treatment-naïve patients with pathologically confirmed lung cancer and twenty healthy subjects were profiled for miRNAs expression. Selected microRNAs were further validated, using quantitative-PCR, in an independent set of 10 subjects from both groups.
A total of 78 miRNAs were found to be significantly upregulated in the EBC of lung cancer patients compared to the control group. Six of these 78 miRNAs were shortlisted for validation. Of these, miR-31-3p, let7i, and miR-449c were significantly upregulated, exhibited good discriminatory power.
Differential expression of miRNAs secreted by lung cells could be quantitated in EBC samples, and could be used as a potential non-invasive tool for early diagnosis of lung cancer.
摘要：
背景：肺细胞分泌的微小RNA的定量可以提供关于肺健康的有价值的信息。呼气冷凝液（EBC）提供了一种非侵入性的方式来采样分泌的microRNAs，并可用作肺癌的诊断工具。
方法：对来自20名未治疗的肺癌患者和20名健康受试者的EBC样品的miRNAs表达进行分析。选择的microRNA进一步验证，使用定量PCR，在两组10名受试者的独立集合中。
结果：发现与对照组相比，肺癌患者的EBC中总共有78个miRNAs显著上调。这78个miRNA中的6个入围用于验证。其中,miR-31-3p,let7i,和miR-449c显著上调，表现出良好的歧视能力。
结论：肺细胞分泌的miRNAs的差异表达可以在EBC样本中定量，并可作为肺癌早期诊断的潜在非侵入性工具。
方法：对来自20名未治疗的肺癌患者和20名健康受试者的EBC样品的miRNAs表达进行分析。
结果：发现与对照组相比，肺癌患者的EBC中总共有78个miRNAs显著上调。
结论：肺细胞分泌的miRNAs的差异表达可以在EBC样本中定量，
