Abstract：
Epstein Barr Virus (EBV) is implicated in the carcinogenesis of nasopharyngeal carcinoma (NPC) and currently associated with at least 1% of global cancers. The differential prognosis analysis of NPC in EBV genotypes remains to be elucidated. Medical, radiological, pathological, and laboratory reports of 146 NPC patients were collected retrospectively over a 6-year period between 2015 and 2020. From the pathology archives, DNA was extracted from tumor blocks and used for EBV nuclear antigen 3C (EBNA-3C) genotyping by nested polymerase chain reaction (PCR). We found a high prevalence of 96% of EBV infection in NPC patients with a predominance of genotype I detected in 73% of NPC samples. Histopathological examination showed that most of the NPC patients were in the advanced stages of cancer: stage III (38.4%) or stage IV-B (37.7%). Only keratinized squamous cell carcinoma was significantly higher in EBV negative NPC patients compared with those who were EBV positive (OR = 0.01, 95%CI = (0.004-0.32; p = 0.009)), whereas the majority of patients (91.8%) had undifferentiated, non-keratinizing squamous cell carcinoma, followed by differentiated, non-keratinizing squamous cell carcinoma (7.5%). Although NPC had metastasized to 16% of other body sites, it was not associated with EBV infection, except for lung metastasis. A statistically significant reverse association was observed between EBV infection and lung metastasis (OR = 0.07, 95%CI = (0.01-0.51; p = 0.008)). Although 13% of NPC patients died, the overall survival (OS) mean time was 5.59 years. Given the high prevalence of EBV-associated NPC in our population, Saudi could be considered as an area with a high incidence of EBV-associated NPC with a predominance of EBV genotype I. A future multi-center study with a larger sample size is needed to assess the true burden of EBV-associated NPC in Saudi Arabia.
摘要：
EB病毒（EBV）与鼻咽癌（NPC）的发生有关，目前与至少1％的全球癌症有关。EBV基因型中NPC的差异预后分析仍有待阐明。医疗，放射学,病态,我们回顾性收集了2015年至2020年6年间146例NPC患者的实验室报告.从病理学档案中，从肿瘤块中提取DNA，并通过巢式聚合酶链反应（PCR）用于EBV核抗原3C（EBNA-3C）基因分型。我们发现NPC患者中96％的EBV感染率很高，在73％的NPC样本中检测到基因型I占主导地位。组织病理学检查显示，大多数NPC患者处于癌症的晚期阶段：III期（38.4％）或IV-B期（37.7％）。与EBV阳性患者相比，EBV阴性NPC患者中只有角化鳞状细胞癌明显更高（OR=0.01，95CI=（0.004-0.32；p=0.009）），而大多数患者（91.8%）未分化，非角化鳞状细胞癌，其次是差异化，非角化性鳞状细胞癌（7.5%）。尽管NPC已经转移到其他身体部位的16％，它与EBV感染无关，除了肺转移。在EBV感染和肺转移之间观察到统计学上显著的反向关联(OR=0.07,95CI=(0.01-0.51;p=0.008))。尽管13%的NPC患者死亡，总生存期(OS)平均时间为5.59年.鉴于EBV相关的NPC在我们人群中的高患病率，沙特可以被认为是EBV相关NPC发病率高的地区，EBV基因型I占主导地位。未来需要更大样本量的多中心研究来评估EBV相关NPC在沙特阿拉伯的真实负担。
