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Abstract：
Background: Lycium barbarum (L. barbarum), popularly referred to as Goji berry, is a promising herb known for its powerful anti-antioxidant, antibacterial, and anti-inflammatory properties. It is used in traditional Chinese medicine for treating inflammatory and infectious diseases. It has also shown good anti-cancer properties and has been tested against liver, colon, prostate, breast, and cervical cancers. However, no study has yet evaluated the role of goji berries against oral cancer. Hence, the present paper aims to evaluate the anticancer properties of L. barbarum against oral squamous cell carcinoma. Method: Ethanolic extract of L. barbarum (EELB) was tested for its anticancer properties by performing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, colony formation, cell proliferation, and scratch wound test. The impact of EELB on the signaling transduction pathways of Extracellular signal-regulated kinase (ERK1/2), protein kinase (AKT1), cyclin D1 and epithelial-mesenchymal transition (EMT) was also assessed by western blot. Results: The results showed that EELB can impede CAL-27 cell growth, proliferation and migration in-vitro. It even reduced the phosphorylation of ERK1/2 and AKT1 with concomitant downregulation of cyclin D1 (CCND1), cadherin 2 (CDH2), and vimentin (VIM) and upregulation of cadherin 1 (CDH1) expression suggesting its anti-proliferative and anti-EMT effects in oral cancer. Conclusion: Goji berry has good antiproliferative and anti-invasive properties. It affects potential EMT markers and signaling transduction pathways involved in oral cancers. Hence goji berry can be tried as a potential anticancer agent to manage oral squamous cell carcinoma.
摘要：
背景：枸杞（L.barbarum),通常被称为Gojiberry，是一种有前途的草药，以其强大的抗抗氧化剂而闻名，抗菌，和抗炎特性。由于其强大的抗氧化剂，它被用于中药，抗菌，和抗炎特性。它还显示出良好的抗癌特性，并已通过肝脏测试，结肠,前列腺,乳房,和宫颈癌。然而,尚未有研究评估枸杞对口腔癌的作用。因此，本文旨在评估L.barbarum对口腔鳞状细胞癌的抗癌特性。方法：通过进行3-（4,5-二甲基噻唑-2-基）-2,5-二苯基-2H-四唑溴化物（MTT）测定，测试了L.barbarum（EELB）的乙醇提取物的抗癌特性，菌落形成,细胞增殖，和划痕测试。EELB对胞外信号调节激酶(ERK1/2)信号转导通路的影响,蛋白激酶（AKT1），细胞周期蛋白D1和上皮间质转化（EMT）也通过蛋白质印迹评估。结果:EELB能抑制CAL-27细胞的生长,体外增殖和迁移。.它甚至减少了ERK1/2和AKT1的磷酸化，伴随着细胞周期蛋白D1（CCND1）的下调，钙粘蛋白2（CDH2），波形蛋白（VIM）和钙粘蛋白1（CDH1）表达上调表明其在口腔癌中的抗增殖和抗EMT作用。结论:枸杞具有良好的抗增殖和抗侵袭性。它影响口腔癌中潜在的EMT标记和信号转导途径。因此，枸杞可以作为一种潜在的抗癌剂来治疗口腔鳞状细胞癌。
