Abstract：
The aim of this study was to evaluate and select entomopathogenic fungi that produces insecticidal compounds for the control of adults of Anastrepha obliqua Macquart (Diptera: tephritidae) that are the main pest of mango (Mangifera indica L. Bark) in Colombia. Nine entomopathogenic fungi isolates were evaluated, five belonging to the genus Metarhizium and four belonging to the genus Beauveria. One strain of the species Metarhizium robertsii with insecticidal activity was selected. By column fractionation, an active fraction was obtained, which caused mortalities higher than 90% after 48 h of exposure. Through HPLC it was determined that the active fraction is composed of more than 22 metabolites. Identification of the metabolites by UHPLC MS/MS revealed the presence of destruxin in E, D, A and B groups (destruxin E-diol, destruxin D, destruxin D1, destruxin D2, destruxin A2, destruxin A, destruxin A3, dihydrodestruxin A, desmB, destruxin B2, destruxin B and destruxin B1). The evaluation of the insecticidal capacity of the organic fractions obtained by HPLC indicated that the extract obtained from the isolate M. robertsii had a compound with high activity on adults of A. obliqua (destruxin A) causing massive mortality of up to 100%, after 48 h of the treatment administration. Furthermore, two other compounds with medium activity were found (destruxin A2 and destruxin B), showing mortalities between 60.0 and 81.3%, respectively. The extract of the isolate MT008 of M. robertsii showed higher insecticidal activity and a potential source for the control of A. obliqua.
摘要：
这项研究的目的是评估和选择产生杀虫化合物的昆虫病原真菌，以控制哥伦比亚芒果（MangiferaindicaL.Bark）的主要害虫。对9个昆虫病原真菌分离株进行了评估，五个属于绿僵菌属，四个属于白僵菌属。选择了一种具有杀虫活性的绿僵菌。通过柱分馏，获得了活性部分，暴露48小时后死亡率高于90%。通过HPLC确定活性部分由超过22种代谢物组成。通过UHPLCMS/MS鉴定代谢物揭示了大肠杆菌中存在destruxin，D,A和B组(去酯素E-二醇，destruxinD,destruxinD1，destruxinD2，destruxinA2，destruxinA，destruxinA3，双氢destruxinA，desmB,destruxinB2、destruxinB和destruxinB1)。对通过HPLC获得的有机部分的杀虫能力的评估表明，从分离的M.robertsii中获得的提取物具有对成虫A（destruxinA）成虫具有高活性的化合物，导致高达100％的大量死亡率。治疗给药后48小时。此外，发现了另外两个具有中等活性的化合物（destruxinA2和destruxinB），死亡率在60.0和81.3%之间，分别。M.robertsii的分离株MT008的提取物显示出更高的杀虫活性，是防治A的潜在来源。
