Abstract：
The emergence of multidrug-resistant (MDR) bacteria has made wound infection treatment difficult, calling for novel strategies for effective elimination of bacteria in wounds and promoting their recovery. Herein, we report a novel chitosan antibacterial sponge combining zinc oxide particles (ZnO) and the photosensitizer chlorin e6 (Ce6), named CS-ZnO/Ce6 sponge for combating multidrug-resistant bacteria and treating skin abscesses. The fabricated CS-ZnO/Ce6 sponge had porous structure with high porosity, conducive to absorbing the wound exudate. Meanwhile, the hemostatic property of this sponge enabled it to stop the continuous bleeding of the wound. Upon 660 nm light irradiation, the CS-ZnO/Ce6 sponge exhibited an instant photodynamic bactericidal effect against several typical MDR strains, and the presence of ZnO could continuously inhibit bacterial growth. In addition, local remedy of methicillin-resistant Staphylococcus aureus (MRSA)-infected mice with CS-ZnO/Ce6 sponge with light irradiation caused a potent immediate bacterial killing effect and prolonged bacteriostasis in mice with skin abscesses, leading to the rapid recovery of the wound. The biocompatibility of the CS-ZnO/Ce6 sponge in mice was also verified by histological examination of the main organs. Collectively, the CS-ZnO/Ce6 sponge with broad-spectrum antibacterial activity and long-term bacterial inhibition potential could be useful for treating microbial infections and accelerating wound healing.
摘要：
多重耐药（MDR）细菌的出现使伤口感染治疗变得困难，呼吁采取新的策略来有效消除伤口中的细菌并促进其恢复。在这里，我们报道了一种新型的壳聚糖抗菌海绵，该海绵结合了氧化锌颗粒（ZnO）和光敏剂二氯酸e6（Ce6），命名为CS-ZnO/Ce6海绵，用于对抗多重耐药细菌和治疗皮肤脓肿。制备的CS-ZnO/Ce6海绵具有高孔隙率的多孔结构,有利于吸收伤口渗出物。同时,这种海绵的止血特性使它能够阻止伤口的持续出血。在660nm的光照射下，CS-ZnO/Ce6海绵对几种典型的MDR菌株具有即时的光动力杀菌作用，ZnO的存在可以持续抑制细菌的生长。此外,用CS-ZnO/Ce6海绵对耐甲氧西林金黄色葡萄球菌（MRSA）感染的小鼠进行局部治疗，并在皮肤脓肿小鼠中产生了有效的立即杀菌效果和长期抑菌效果，导致伤口迅速恢复。CS-ZnO/Ce6海绵在小鼠体内的生物相容性也通过对主要器官的组织学检查得到验证。总的来说，CS-ZnO/Ce6海绵具有广谱的抗菌活性和长期的细菌抑制潜力，可用于治疗微生物感染和促进伤口愈合。
