PDF(Pubmed)
Abstract：
Skin and soft tissue infection (SSTI) caused by atypical mycobacteria such as Mycobacterium abscessus and Mycobacterium avium intracellulare complex (MAIC) have increased in recent years. Current therapeutic options are limited, and hence new and better therapies are urgently required. Colloidal Silver (CS) has been identified for its widespread antibacterial properties and silver-impregnated dressings have been used for SSTIs caused by various pathogens. The efficacy of Green Synthesized Colloidal Silver (GSCS) was investigated for bacterial growth inhibition (BGI) using a microdilution method and minimum biofilm eradication concentration (MBEC) using resazurin assay and confocal scanning laser microscopy (CSLM) of M. abscessus (n = 5) and MAIC (n = 5). The antibacterial effect of GSCS against M. abscessus infected macrophages was also evaluated. The in vitro cytotoxicity of GSCS on a human keratinocyte cell line (HaCaT) and neonatal foreskin fibroblasts was analyzed by the crystal violet proliferation assay. Average BGI and MBEC of GSCS varied between 0.7 and 22 ppm for M. abscessus and MAIC. The concentration of 3 ppm reduced M. abscessus-infection in macrophages significantly. GSCS was not cytotoxic to HaCaT and neonatal foreskin fibroblast cells at concentrations < 3 ppm up to 2 h exposure time. GSCS therefore, has the potential for topical application against atypical mycobacterial SSTI.
摘要：
近年来，由非典型分枝杆菌如脓肿分枝杆菌和鸟分枝杆菌细胞内复合物（MAIC）引起的皮肤和软组织感染（SSTI）有所增加。目前的治疗选择有限，因此迫切需要新的更好的治疗方法。胶体银（CS）因其广泛的抗菌性能而被鉴定，并且浸渍银的敷料已用于由各种病原体引起的SSTI。使用微量稀释方法和最小生物膜根除浓度（MBEC），研究了绿色合成的胶体银（GSCS）对细菌生长抑制（BGI）的功效。脓肿分枝杆菌（n=5）和MAIC（n=5）。还评估了GSCS对脓肿分枝杆菌感染的巨噬细胞的抗菌作用。通过结晶紫增殖测定法分析了GSCS对人角质形成细胞系（HaCaT）和新生儿包皮成纤维细胞的体外细胞毒性。对于脓肿分枝杆菌和MAIC，GSCS的平均BGI和MBEC在0.7至22ppm之间变化。3ppm的浓度显著降低了巨噬细胞中脓肿分枝杆菌的感染。GSCS对HaCaT和新生儿包皮成纤维细胞无细胞毒性,浓度<3ppm直至2小时暴露时间。因此，GSCS，具有针对非典型分枝杆菌SSTI的局部应用潜力。
