Abstract：
This meta-analysis was designed to assess the effect of the addition of a bead-beating (BB) step during DNA extraction to effectively isolate Trichuris trichiura DNA from stool samples for quantitative polymerase chain reaction (qPCR)-based diagnosis. qPCR-based molecular studies comparing the inclusion of a bead-beating step during the DNA extraction from stool samples with extraction without the step were included in the analysis. Studies using real patient samples in community settings were included. The PubMed database and Google search engine were searched in December 2019. Risk of bias and applicability were assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 checklist. Odds ratios (ORs) for individual studies were combined to estimate the random effects model OR. A total of six independent sub-studies were gathered from two published original articles. The division of the two major studies into six sub-studies was indispensable due to the nature of the study carried out. 128 of the total 192 samples (in all studies) were positive for T. trichiura when BB was used during DNA extraction compared to 108/192 when BB was excluded. The combined OR was 1.66 (95% confidence interval: 1.059 to 2.602). Though only two articles were included in the study, six exclusive individual sub-studies were analyzed. Inherent differences in the background prevalence of helminths in the study population could impact the sensitivity of qPCR. It was found that the inclusion of the BB step during DNA extraction significantly increased the sensitivity of the test. This study was not registered in any database.
摘要：
该荟萃分析旨在评估在DNA提取过程中添加打珠（BB）步骤以有效地从粪便样品中分离TrichurisTrichilaDNA以进行基于定量聚合酶链反应（qPCR）的诊断的效果。在分析中包括了基于qPCR的分子研究,比较了在从粪便样品提取DNA过程中包含打珠步骤和没有该步骤的提取。包括在社区环境中使用真实患者样本的研究。PubMed数据库和Google搜索引擎于2019年12月进行了搜索。使用诊断准确性研究质量评估-2清单评估偏倚和适用性的风险。将个体研究的赔率比(OR)组合以估计随机效应模型OR。从两篇已发表的原始文章中收集了总共六个独立的子研究。由于所进行研究的性质，将两项主要研究分为六个子研究是必不可少的。当在DNA提取期间使用BB时,总共192个样品中的128个(在所有研究中)是Trichiura的阳性,而当排除BB时是108/192。合并OR为1.66(95%置信区间:1.059~2.602)。虽然只有两篇文章被纳入研究，分析了六个独家的个体子研究。研究人群中蠕虫背景患病率的固有差异可能会影响qPCR的敏感性。发现在DNA提取过程中包含BB步骤显着提高了测试的灵敏度。这项研究没有在任何数据库中注册。
