The tumor-bearing mouse model was established with MC38 mouse colon cancer cells, and the mice were divided into the control group, tumor-resected group, and tumor-bearing group. Urine was collected 7 and 30 days after tumor resection. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to identify the urine proteome, which was analyzed for differentially expressed proteins and functional annotation.
(1) Seven days after tumor resection, 20 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included circadian rhythm, Notch signaling pathway, leukocyte cell-cell adhesion, and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. (2) Thirty days after tumor resection, 33 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included cell adhesion; complement activation, the alternative pathway; the immune system process; and angiogenesis. (3) The difference in the urine proteome between the tumor-resected group and the healthy control group was smaller 30 days after tumor resection.
Changes in the urinary proteome can reflect the complete resection of MC38 tumors.
UNASSIGNED:用MC38小鼠结肠癌细胞建立荷瘤小鼠模型,将小鼠分为对照组,肿瘤切除组,和荷瘤组。在肿瘤切除后7天和30天收集尿液。采用液相色谱-串联质谱(LC-MS/MS)对尿蛋白组进行鉴定,分析了差异表达的蛋白质和功能注释。
未经批准:(1)肿瘤切除后7天,20种差异表达的蛋白质区分了肿瘤切除组和荷瘤组。确定的生物过程包括昼夜节律,Notch信号通路,白细胞-细胞粘附,和通过质膜细胞粘附分子的异源性细胞-细胞粘附。(2)肿瘤切除后30天,33种差异表达的蛋白质区分了肿瘤切除组和荷瘤组。确定的生物过程包括细胞粘附;补体激活,替代途径;免疫系统过程;和血管生成。(3)肿瘤切除后30天,肿瘤切除组与健康对照组之间的尿液蛋白质组差异较小。
UNASSIGNED:尿蛋白质组的变化可以反映MC38肿瘤的完全切除。