PDF(Pubmed)
Abstract：
This study aimed to address on the most important concern of surgeons-whether to completely resect tumor. Urine can indicate early changes associated with physiological or pathophysiological processes. Based on these ideas, we conducted experiments to explore changes in the urine proteome between tumor-bearing mice and tumor-resected mice.
The tumor-bearing mouse model was established with MC38 mouse colon cancer cells, and the mice were divided into the control group, tumor-resected group, and tumor-bearing group. Urine was collected 7 and 30 days after tumor resection. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to identify the urine proteome, which was analyzed for differentially expressed proteins and functional annotation.
(1) Seven days after tumor resection, 20 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included circadian rhythm, Notch signaling pathway, leukocyte cell-cell adhesion, and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. (2) Thirty days after tumor resection, 33 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included cell adhesion; complement activation, the alternative pathway; the immune system process; and angiogenesis. (3) The difference in the urine proteome between the tumor-resected group and the healthy control group was smaller 30 days after tumor resection.
Changes in the urinary proteome can reflect the complete resection of MC38 tumors.
The tumor-bearing mouse model was established with MC38 mouse colon cancer cells, and the mice were divided into the control group, tumor-resected group, and tumor-bearing group. Urine was collected 7 and 30 days after tumor resection. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to identify the urine proteome, which was analyzed for differentially expressed proteins and functional annotation.
(1) Seven days after tumor resection, 20 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included circadian rhythm, Notch signaling pathway, leukocyte cell-cell adhesion, and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. (2) Thirty days after tumor resection, 33 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included cell adhesion; complement activation, the alternative pathway; the immune system process; and angiogenesis. (3) The difference in the urine proteome between the tumor-resected group and the healthy control group was smaller 30 days after tumor resection.
Changes in the urinary proteome can reflect the complete resection of MC38 tumors.
摘要：
UNASSIGNED：这项研究旨在解决外科医生最重要的问题-是否完全切除肿瘤。尿液可以指示与生理或病理生理过程相关的早期变化。基于这些想法,我们进行了实验，以探讨荷瘤小鼠和肿瘤切除小鼠之间尿蛋白组的变化。
UNASSIGNED:用MC38小鼠结肠癌细胞建立荷瘤小鼠模型，将小鼠分为对照组，肿瘤切除组，和荷瘤组。在肿瘤切除后7天和30天收集尿液。采用液相色谱-串联质谱(LC-MS/MS)对尿蛋白组进行鉴定,分析了差异表达的蛋白质和功能注释。
未经批准：(1)肿瘤切除后7天，20种差异表达的蛋白质区分了肿瘤切除组和荷瘤组。确定的生物过程包括昼夜节律，Notch信号通路,白细胞-细胞粘附，和通过质膜细胞粘附分子的异源性细胞-细胞粘附。（2）肿瘤切除后30天，33种差异表达的蛋白质区分了肿瘤切除组和荷瘤组。确定的生物过程包括细胞粘附；补体激活，替代途径；免疫系统过程；和血管生成。（3）肿瘤切除后30天，肿瘤切除组与健康对照组之间的尿液蛋白质组差异较小。
UNASSIGNED：尿蛋白质组的变化可以反映MC38肿瘤的完全切除。
UNASSIGNED:用MC38小鼠结肠癌细胞建立荷瘤小鼠模型，将小鼠分为对照组，肿瘤切除组，和荷瘤组。在肿瘤切除后7天和30天收集尿液。采用液相色谱-串联质谱(LC-MS/MS)对尿蛋白组进行鉴定,分析了差异表达的蛋白质和功能注释。
未经批准：(1)肿瘤切除后7天，20种差异表达的蛋白质区分了肿瘤切除组和荷瘤组。确定的生物过程包括昼夜节律，Notch信号通路,白细胞-细胞粘附，和通过质膜细胞粘附分子的异源性细胞-细胞粘附。（2）肿瘤切除后30天，33种差异表达的蛋白质区分了肿瘤切除组和荷瘤组。确定的生物过程包括细胞粘附；补体激活，替代途径；免疫系统过程；和血管生成。（3）肿瘤切除后30天，肿瘤切除组与健康对照组之间的尿液蛋白质组差异较小。
UNASSIGNED：尿蛋白质组的变化可以反映MC38肿瘤的完全切除。
