Abstract：
BACKGROUND: Acne is the eighth-most prevalent inflammatory skin disease with no optimal treatment. Photodynamic therapy (PDT) is an effective treatment for severe acne.
OBJECTIVE: The effect of PDT on the composition and diversity of skin microflora in severe acne patients was studied.
METHODS: A total of 18 patients with severe acne and 8 healthy individuals were selected for this study. Patients were treated with 5-aminolevulinic acid-mediated PDT once a week three times in total; the skin microbiome was measured by 16S ribosomal RNA gene sequencing before and after treatment (1 week after each PDT).
RESULTS: The microflora composition was different between healthy controls and patients, and between patients before and after treatment. Alpha diversity indices were lower in patients than those in control. There were 15 bacterial genera with high relative abundance that had noticeable changes during treatment. At the genus level,particularly Cutibacterium acnes (C. acnes formerly Propionibacterium acnes), there was no statistically significant difference among different group. The abundances of Staphylococcus epidermidis and Staphylococcus aureus were low.
CONCLUSIONS: The microbial composition is different between severe acne patients acne patients and healthy individuals. The therapeutic efficacy of severe acne treated with PDT is associated with the composition and diversity of skin microbiota.
CONCLUSIONS: The skin microbial composition changes after PDT treatment. PDT is an effective method for the treatment of severe acne.
OBJECTIVE: The effect of PDT on the composition and diversity of skin microflora in severe acne patients was studied.
METHODS: A total of 18 patients with severe acne and 8 healthy individuals were selected for this study. Patients were treated with 5-aminolevulinic acid-mediated PDT once a week three times in total; the skin microbiome was measured by 16S ribosomal RNA gene sequencing before and after treatment (1 week after each PDT).
RESULTS: The microflora composition was different between healthy controls and patients, and between patients before and after treatment. Alpha diversity indices were lower in patients than those in control. There were 15 bacterial genera with high relative abundance that had noticeable changes during treatment. At the genus level,particularly Cutibacterium acnes (C. acnes formerly Propionibacterium acnes), there was no statistically significant difference among different group. The abundances of Staphylococcus epidermidis and Staphylococcus aureus were low.
CONCLUSIONS: The microbial composition is different between severe acne patients acne patients and healthy individuals. The therapeutic efficacy of severe acne treated with PDT is associated with the composition and diversity of skin microbiota.
CONCLUSIONS: The skin microbial composition changes after PDT treatment. PDT is an effective method for the treatment of severe acne.
摘要：
背景：痤疮是第八大流行的炎症性皮肤病，没有最佳治疗方法。光动力疗法（PDT）是严重痤疮的有效治疗方法。
目的：研究了PDT对重度痤疮患者皮肤菌群组成和多样性的影响。
方法：选择18例重度痤疮患者和8例健康个体进行本研究。患者接受5-氨基乙酰丙酸介导的PDT治疗，每周一次，共三次；治疗前后（每次PDT后1周）通过16S核糖体RNA基因测序测量皮肤微生物组。
结果：健康对照组和患者的微生物组成不同，以及治疗前后的患者之间。患者的α多样性指数低于对照组。有15个相对丰度较高的细菌属，在治疗过程中有明显的变化。在属一级，特别是痤疮切杆菌(C.痤疮原痤疮丙酸杆菌)，各组间差异无统计学意义。表皮葡萄球菌和金黄色葡萄球菌的丰度较低。
结论：重度痤疮患者和健康个体的微生物组成不同。用PDT治疗的严重痤疮的治疗功效与皮肤微生物群的组成和多样性有关。
结论：PDT治疗后皮肤微生物组成发生变化。PDT是治疗严重痤疮的有效方法。
目的：研究了PDT对重度痤疮患者皮肤菌群组成和多样性的影响。
方法：选择18例重度痤疮患者和8例健康个体进行本研究。患者接受5-氨基乙酰丙酸介导的PDT治疗，每周一次，共三次；治疗前后（每次PDT后1周）通过16S核糖体RNA基因测序测量皮肤微生物组。
结果：健康对照组和患者的微生物组成不同，以及治疗前后的患者之间。患者的α多样性指数低于对照组。有15个相对丰度较高的细菌属，在治疗过程中有明显的变化。在属一级，特别是痤疮切杆菌(C.痤疮原痤疮丙酸杆菌)，各组间差异无统计学意义。表皮葡萄球菌和金黄色葡萄球菌的丰度较低。
结论：重度痤疮患者和健康个体的微生物组成不同。用PDT治疗的严重痤疮的治疗功效与皮肤微生物群的组成和多样性有关。
结论：PDT治疗后皮肤微生物组成发生变化。PDT是治疗严重痤疮的有效方法。
