Abstract：
BACKGROUND: Trichosporon asahii is an opportunistic pathogenic yeast-like fungus. Phospholipase B1 (PLB1) is an important virulence factor of pathogenic fungi such as Candida albicans and Cryptococcus neoformans, and there are few studies on the role of PLB1 in the pathogenicity of T. asahii.
OBJECTIVE: To investigate the role of PLB1 in the pathogenicity of T. asahii.
METHODS: A strain with low secretion of PLB1 (4848) was screened, a PLB1 overexpression strain (PLB1OX ) was constructed, and the differences in histopathology, fungal load of organ, survival time of mice, the levels of IL-6, IL-10, TNF-α, and GM-GSF in the serum and organs caused by the two strains were compared.
RESULTS: Histopathology showed that spores and hyphae were observed in both groups, and PLB1OX led to more fungal invasion. The fungal loads in the kidney, lung, spleen and liver in the PLB1OX group were significantly higher than those in the 4848 group, and the survival time of mice was significantly lower than that in the 4848 group. The levels of TNF-α in the serum, liver, spleen, lung and kidney of the PLB1OX group were lower than those of the 4848 group, while the level of IL-10 in the serum was higher than that of the 4848 group.
CONCLUSIONS: These results suggest that PLB1 can enhance the invasive function of T. asahii and affect the secretion of TNF-α and IL-10 which may affect the host antifungal immune response, providing evidence that PLB1 plays a role in the pathogenic infection of T. asahii.
OBJECTIVE: To investigate the role of PLB1 in the pathogenicity of T. asahii.
METHODS: A strain with low secretion of PLB1 (4848) was screened, a PLB1 overexpression strain (PLB1OX ) was constructed, and the differences in histopathology, fungal load of organ, survival time of mice, the levels of IL-6, IL-10, TNF-α, and GM-GSF in the serum and organs caused by the two strains were compared.
RESULTS: Histopathology showed that spores and hyphae were observed in both groups, and PLB1OX led to more fungal invasion. The fungal loads in the kidney, lung, spleen and liver in the PLB1OX group were significantly higher than those in the 4848 group, and the survival time of mice was significantly lower than that in the 4848 group. The levels of TNF-α in the serum, liver, spleen, lung and kidney of the PLB1OX group were lower than those of the 4848 group, while the level of IL-10 in the serum was higher than that of the 4848 group.
CONCLUSIONS: These results suggest that PLB1 can enhance the invasive function of T. asahii and affect the secretion of TNF-α and IL-10 which may affect the host antifungal immune response, providing evidence that PLB1 plays a role in the pathogenic infection of T. asahii.
摘要：
背景：asahii曲孢菌是一种机会致病性酵母样真菌。磷脂酶B1(PLB1)是白色念珠菌和新生隐球菌等病原真菌的重要毒力因子,而PLB1在麻黄致病性中的作用研究较少。
目的：探讨PLB1在大麻黄致病性中的作用。
方法：筛选PLB1（4848）分泌少的菌株，构建了PLB1过表达菌株（PLB1OX），以及组织病理学的差异，器官的真菌负荷，小鼠的存活时间,比较两种菌株在血清和器官中IL-6、IL-10、TNF-α和GM-GSF的水平。
结果：组织病理学显示两组均有孢子和菌丝，PLB1OX导致更多的真菌入侵。肾脏中的真菌负荷，肺，PLB1OX组的脾脏和肝脏明显高于4848组，小鼠存活时间明显低于4848组。血清中TNF-α水平,肝脏,脾,脾PLB1OX组的肺和肾低于4848组,而血清中IL-10水平高于4848组。
结论：这些结果表明，PLB1可以增强青芝的侵袭功能，并影响TNF-α和IL-10的分泌，这可能会影响宿主的抗真菌免疫反应。提供PLB1在T.asahii致病性感染中起作用的证据。
目的：探讨PLB1在大麻黄致病性中的作用。
方法：筛选PLB1（4848）分泌少的菌株，构建了PLB1过表达菌株（PLB1OX），以及组织病理学的差异，器官的真菌负荷，小鼠的存活时间,比较两种菌株在血清和器官中IL-6、IL-10、TNF-α和GM-GSF的水平。
结果：组织病理学显示两组均有孢子和菌丝，PLB1OX导致更多的真菌入侵。肾脏中的真菌负荷，肺，PLB1OX组的脾脏和肝脏明显高于4848组，小鼠存活时间明显低于4848组。血清中TNF-α水平,肝脏,脾,脾PLB1OX组的肺和肾低于4848组,而血清中IL-10水平高于4848组。
结论：这些结果表明，PLB1可以增强青芝的侵袭功能，并影响TNF-α和IL-10的分泌，这可能会影响宿主的抗真菌免疫反应。提供PLB1在T.asahii致病性感染中起作用的证据。
