Abstract：
To evaluate the association of SYN2, PPARG, RAF1, TIMP4, and IQSEC1 polymorphisms in 3p25 with oral squamous cell carcinoma (OSCC) in the Chinese Han population.
Genomic DNA was extracted from 494 subjects with or without OSCC. Basic information on the subjects, clinical data, and prognoses were collected. Fifteen candidate single nucleotide polymorphisms (SNPs) were selected and genotyped. The statistical analyses included descriptive statistics, logistic regression, survival, and functional annotation was performed.
IQSEC1-rs2686742 correlated with OSCC occurrence. In addition, RAF1-rs1051208, PPARG-rs10865710, PPARG-rs3856806, IQSEC1-rs2686742, PPARG-rs1175544, IQSEC1-rs9211, and IQSEC1-rs2600322 were significantly associated with the clinical characteristics of patients with OSCC. The log-rank test showed that IQSEC1-rs2600322 may play an important role in the survival of patients with OSCC. The Cox regression analysis suggested that PPARG-rs10865710, PPARG-rs7649970, IQSEC1-rs9211, IQSEC1-rs2600322, and IQSEC1-rs12487715 influenced survival outcomes. The functional annotation indicated that the transcript levels of IQSEC1 were upregulated in head and neck squamous cell carcinoma tissues, whereas PPARG gene transcription was downregulated.
IQSEC1-rs2686742 may be closely associated with OSCC onset. Multiple SNPs in IQSEC1 and PPARG genes correlated with the clinical characteristics of OSCC, among which PPARG-rs10865710, IQSEC1-rs9211, and IQSEC1-rs2600322 were associated with cancer prognosis.
Genomic DNA was extracted from 494 subjects with or without OSCC. Basic information on the subjects, clinical data, and prognoses were collected. Fifteen candidate single nucleotide polymorphisms (SNPs) were selected and genotyped. The statistical analyses included descriptive statistics, logistic regression, survival, and functional annotation was performed.
IQSEC1-rs2686742 correlated with OSCC occurrence. In addition, RAF1-rs1051208, PPARG-rs10865710, PPARG-rs3856806, IQSEC1-rs2686742, PPARG-rs1175544, IQSEC1-rs9211, and IQSEC1-rs2600322 were significantly associated with the clinical characteristics of patients with OSCC. The log-rank test showed that IQSEC1-rs2600322 may play an important role in the survival of patients with OSCC. The Cox regression analysis suggested that PPARG-rs10865710, PPARG-rs7649970, IQSEC1-rs9211, IQSEC1-rs2600322, and IQSEC1-rs12487715 influenced survival outcomes. The functional annotation indicated that the transcript levels of IQSEC1 were upregulated in head and neck squamous cell carcinoma tissues, whereas PPARG gene transcription was downregulated.
IQSEC1-rs2686742 may be closely associated with OSCC onset. Multiple SNPs in IQSEC1 and PPARG genes correlated with the clinical characteristics of OSCC, among which PPARG-rs10865710, IQSEC1-rs9211, and IQSEC1-rs2600322 were associated with cancer prognosis.
摘要：
目的：评估SYN2、PPARG、中国汉族人群口腔鳞状细胞癌（OSCC）3p25中RAF1，TIMP4和IQSEC1多态性。
方法：从494名有或没有OSCC的受试者中提取基因组DNA。关于主题的基本信息，临床资料,并收集了预后。选择15个候选单核苷酸多态性（SNP）并进行基因分型。统计分析包括描述性统计，逻辑回归，生存,并进行功能注释。
结果：IQSEC1-rs2686742与OSCC发生相关。此外,RAF1-rs1051208、PPARG-rs10865710、PPARG-rs3856806、IQSEC1-rs2686742、PPARG-rs1175544、IQSEC1-rs9211和IQSEC1-rs2600322与OSCC患者的临床特征显著相关。对数秩检验显示IQSEC1-rs2600322可能在OSCC患者的生存中起重要作用。Cox回归分析提示PPARG-rs10865710、PPARG-rs7649970、IQSEC1-rs9211、IQSEC1-rs2600322和IQSEC1-rs12487715影响生存结局。功能注释表明头颈部鳞状细胞癌组织中IQSEC1的转录水平上调，而PPARG基因转录下调。
结论：IQSEC1-rs2686742可能与OSCC发病密切相关。IQSEC1和PPARG基因中的多个SNPs与OSCC的临床特征相关,其中PPARG-rs10865710,IQSEC1-rs9211和IQSEC1-rs2600322与癌症预后相关.
方法：从494名有或没有OSCC的受试者中提取基因组DNA。关于主题的基本信息，临床资料,并收集了预后。选择15个候选单核苷酸多态性（SNP）并进行基因分型。统计分析包括描述性统计，逻辑回归，生存,并进行功能注释。
结果：IQSEC1-rs2686742与OSCC发生相关。此外,RAF1-rs1051208、PPARG-rs10865710、PPARG-rs3856806、IQSEC1-rs2686742、PPARG-rs1175544、IQSEC1-rs9211和IQSEC1-rs2600322与OSCC患者的临床特征显著相关。对数秩检验显示IQSEC1-rs2600322可能在OSCC患者的生存中起重要作用。Cox回归分析提示PPARG-rs10865710、PPARG-rs7649970、IQSEC1-rs9211、IQSEC1-rs2600322和IQSEC1-rs12487715影响生存结局。功能注释表明头颈部鳞状细胞癌组织中IQSEC1的转录水平上调，而PPARG基因转录下调。
结论：IQSEC1-rs2686742可能与OSCC发病密切相关。IQSEC1和PPARG基因中的多个SNPs与OSCC的临床特征相关,其中PPARG-rs10865710,IQSEC1-rs9211和IQSEC1-rs2600322与癌症预后相关.
