Abstract：
Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a causative agent of numerous chronic diseases, including caseous lymphadenitis (CLA) in sheep and goats, which has a zoonotic potential in humans in addition to a poor therapeutic response. In this study, out of 120 collected samples, only 12 (10%) were positive for C. pseudotuberculosis by PCR and by intraperitoneal injection of male Guinea pigs and then characterized for antimicrobial susceptibility and its genetic-relatedness by enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), which showed 2-4 bands ranging from 100 to 3000 bp that can be clustered into four clusters (C1-C4). Despite the serotype biovar 1 only infecting sheep and goats, ERIC-PCR reveals intra-subtyping variation. Examination of affected LNs and organs revealed marked enlargement with either thick creamy green pus or multiple abscesses of variable sizes with a central caseated core surrounded by dense fibrous capsule. A histopathological examination revealed a central necrotic core surrounded by a peripheral mantle of mononuclear cells and a fibrous capsule. Positive immune expression of nuclear factor kappa B (NF-κB/p65) and interleukin-1β (IL-1β) and negative expression of tumor necrosis factor (TNF) in CLA is the first report to our knowledge. Conclusion: In CLA pyogranulomas, IL1β is a more crucial proinflammatory cytokine than TNF in the regulation of C. pseudotuberculosis infection, which is accompanied by marked NF-κB immunoexpression. Therefore, the NF-κB/p65 signaling pathway is involved in the activation of IL1β, and additional immunohistochemical studies are required to determine the various roles of NF-κB/p65 in the inflammatory response within CLA pyogranulomas to control this pathogen.
摘要：
假结核棒状杆菌（C.假性结核病）是许多慢性疾病的病原体，包括绵羊和山羊的干酪性淋巴结炎（CLA），除了较差的治疗反应外，它在人类中还具有人畜共患病的潜力。在这项研究中,在收集的120个样本中,通过PCR和腹膜内注射雄性豚鼠，只有12例（10％）假结核分枝杆菌呈阳性，然后通过肠杆菌重复基因间共有聚合酶链反应（ERIC-PCR）表征抗菌药物敏感性及其遗传相关性，显示2-4个从100到3000bp的条带，可以聚类成四个簇（C1-C4）。尽管血清型biovar1只感染绵羊和山羊，ERIC-PCR显示亚型内变异。对受影响的LN和器官的检查显示，明显肿大，有浓稠的乳状绿色脓液或多个大小可变的脓肿，中央的酪蛋白状核心被致密的纤维囊包围。组织病理学检查显示，中央坏死核心被单核细胞和纤维囊的外周膜包围。CLA中核因子κB（NF-κB/p65）和白细胞介素1β（IL-1β）的阳性免疫表达和肿瘤坏死因子（TNF）的阴性表达是我们所知的首次报道。结论：在CLA脓性肉芽肿中，IL1β是比TNF更重要的促炎细胞因子，在假结核分枝杆菌感染的调节中，伴随着明显的NF-κB免疫表达。因此,NF-κB/p65信号通路参与IL1β的激活,需要进行其他免疫组织化学研究，以确定NF-κB/p65在CLA脓性肉芽肿炎症反应中的各种作用，以控制这种病原体。
