Abstract：
The aim of the study was to verify the quality of ram semen, frozen in 1982-1983, from the historical collection of the Bank of Biological Material of the National Research Institute of Animal Production. A total of 18 ejaculates from 3 Świniarka type rams were analyzed to assess sperm motility (subjectively), total motility, progressive motility, sperm concentration (CASA), membrane integrity (SYBR-14/PI) and chromatin structure (SCSA). In order to determine sperm fertilizing ability 49 ewes were intracervically inseminated (200×106 sperm per AI) with frozen- thawed semen 12 and 24 hours after detection of estrus. Sperm motility parameters, membrane intact spermatozoa and DFI did not differ among the analyzed rams. Spermatozoa concentration was significantly higher for ram no. 2 than for rams no. 1 and 3. The lambing rates (27.3 to 36.0%) did not differ significantly for individual rams. The ram semen, which had been stored for around 40 years, showed satisfactory quality and fertilizing capacity, allowing for its use in artificial insemination.
摘要：
该研究的目的是验证RAM精液的质量，1982年至1983年，从国家动物生产研究所生物材料银行的历史收藏中冻结。共分析了3只winiarka型公羊的18种射精，以评估精子活力（主观），总运动性，渐进运动，精子浓度（CASA），膜完整性(SYBR-14/PI)和染色质结构(SCSA)。为了确定精子受精能力，在检测到发情后12和24小时，用冻融的精液对49只母羊进行了体内授精（每个AI200×106个精子）。精子运动参数,膜完整精子和DFI在分析的公羊之间没有差异。公羊的精子浓度明显更高。2比公羊没有。1和3。各个公羊的羔羊率（27.3％至36.0％）没有显着差异。RAM精液，已经储存了大约40年，表现出令人满意的质量和施肥能力，允许其用于人工授精。
