Abstract：
Spina bifida is one of the neural tube defects, with a high incidence in human birth defects, which seriously affects the health and quality of life of patients. In the treatment of bone defects, the source of autologous bone is limited and will cause secondary damage to the patient. At the same time, since the bone tissue in animals needs to play a variety of biological functions, its complex structure cannot be replaced by a single material. The combination of mechanical materials and biological materials has become a common choice. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have the advantages of easy access, rapid proliferation, low immunogenicity, and no ethical issues. It is often used in the clinical research of tissue regeneration and repair. Therefore, in this study, we established a spina bifida model using Japanese white rabbits. This model was used to screen the best regenerative repair products for congenital spina bifida, and to evaluate the safety of regenerative repair products. The results showed that the combination of hUC-MSCs with collagen material had better regeneration effect than collagen material alone, and had no negative impact on the health of animals. This study provides a new idea for the clinical treatment of spina bifida, and also helps to speed up the research progress of regenerative repair products.
摘要：
脊柱裂是神经管缺陷之一,人类出生缺陷的发病率很高，严重影响患者的健康和生活质量。在骨缺损的治疗中，自体骨来源有限，会对患者造成二次损伤。同时,由于动物的骨组织需要发挥各种生物学功能，其复杂的结构是单一材料所不能替代的。机械材料与生物材料的结合已成为一种普遍的选择。人脐带间充质干细胞(hUC-MSCs)具有易获取、迅速扩散,低免疫原性,也没有道德问题.在组织再生修复的临床研讨中经常使用。因此,在这项研究中，我们用日本大白兔建立了脊柱裂模型。该模型用于筛选先天性脊柱裂的最佳再生修复产品，评估再生修复产品的安全性。结果表明，hUC-MSCs与胶原质材料联合使用比单独使用胶原质材料具有更好的再生效果。对动物的健康没有负面影响。本研究为脊柱裂的临床治疗提供了新的思路,也有助于加快再生修复产品的研究进展。
