PDF(Pubmed)
Abstract：
UNASSIGNED: Multiple irrigation solutions are used in orthopedic surgeries although there are limited studies on their lasting effects on human tissues. The purpose of this work was to investigate the cytotoxic effects of the irrigation solutions Bacitracin, Clorpactin (sodium oxychlorosene), Irrisept (0.05% chlorhexidine gluconate), and Bactisure (ethanol 1%, acetic acid 0.6%, sodium acetate 0.2%, benzalkonium chloride 0.013%, and water) on 3D cultures of human fibroblasts.
UNASSIGNED: Two independent experiments with 6 replicates were performed for the following conditions: Control (saline), bacitracin, Clorpactin, Irrisept, and Bactisure. Human fibroblast cell sheets were exposed to these solutions (1 or 2 min), followed by three washes with warm saline. Cell sheets were then cultured for additional 5- and 7-day posttreatment. Cell viability was measured using the alamarBlue (AB) assay. The more cytotoxic the irrigant, the lower the AB reduction.
UNASSIGNED: For 1-min exposure time, significant differences in AB reduction were noted in Clorpactin, Irrisept, and Bactisure groups compared to control at both 5 days (Clorpactin p = 0.0003, Irrisept p = 7.31 × 10-15, Bactisure p = 6.86 × 10-14) and 7 days posttreatment (all groups p < 0.0001). The results were similar in the 2-min exposure groups. Bacitracin-treated fibroblasts displayed no significant difference at all measurement times compared to control.
UNASSIGNED: Impacts of irrigation solution exposure on cell viability were varied. Irrisept and Bactisure showed the highest cell toxicity even after a brief exposure (1 min), while bacitracin and Clor-pactin exposure showed smaller impacts on cell viability as compared to saline controls. This in vitro study provided insight into the effects of the irrigants on human cells and provides the groundwork essential to move to in vivo studies. Our findings raised the concern that some irrigation solutions may have negative impacts on wound healing and healthy cellular response.
UNASSIGNED: Two independent experiments with 6 replicates were performed for the following conditions: Control (saline), bacitracin, Clorpactin, Irrisept, and Bactisure. Human fibroblast cell sheets were exposed to these solutions (1 or 2 min), followed by three washes with warm saline. Cell sheets were then cultured for additional 5- and 7-day posttreatment. Cell viability was measured using the alamarBlue (AB) assay. The more cytotoxic the irrigant, the lower the AB reduction.
UNASSIGNED: For 1-min exposure time, significant differences in AB reduction were noted in Clorpactin, Irrisept, and Bactisure groups compared to control at both 5 days (Clorpactin p = 0.0003, Irrisept p = 7.31 × 10-15, Bactisure p = 6.86 × 10-14) and 7 days posttreatment (all groups p < 0.0001). The results were similar in the 2-min exposure groups. Bacitracin-treated fibroblasts displayed no significant difference at all measurement times compared to control.
UNASSIGNED: Impacts of irrigation solution exposure on cell viability were varied. Irrisept and Bactisure showed the highest cell toxicity even after a brief exposure (1 min), while bacitracin and Clor-pactin exposure showed smaller impacts on cell viability as compared to saline controls. This in vitro study provided insight into the effects of the irrigants on human cells and provides the groundwork essential to move to in vivo studies. Our findings raised the concern that some irrigation solutions may have negative impacts on wound healing and healthy cellular response.
摘要：
UNASSIGNED：骨科手术中使用了多种冲洗溶液，尽管对其对人体组织的持久影响的研究有限。这项工作的目的是研究灌溉溶液杆菌肽的细胞毒性作用，Clorpactin（羟氯酸钠），Irrisept(0.05%葡萄糖酸氯己定),和Bactsure(乙醇1%，乙酸0.6%，乙酸钠0.2%，苯扎氯铵0.013%,和水）在人成纤维细胞的3D培养物中。
UNASSIGNED：对以下条件进行了两次独立实验，重复6次：对照（盐水），杆菌肽,Clorpactin,Irrisept,还有Bactisure.将人成纤维细胞片暴露于这些溶液（1或2分钟），然后用热盐水洗三次。然后将细胞片培养另外的5天和7天的后处理。使用alamarBlue(AB)测定法测量细胞活力。冲洗剂的细胞毒性越大，AB降低越低。
未经批准：对于1分钟的曝光时间，在Clorpactin中注意到AB降低的显着差异，Irrisept,和Bactisure组在治疗后5天（Clorpactinp=0.0003，Irriseptp=7.31×10-15，Bactisurep=6.86×10-14）和7天（所有组p<0.0001）与对照组相比。2分钟暴露组的结果相似。与对照相比,杆菌肽处理的成纤维细胞在所有测量时间均未显示显著差异。
UNASSIGNED:冲洗溶液暴露对细胞活力的影响是不同的。即使短暂暴露（1分钟），Irrisept和Bactisure也显示出最高的细胞毒性，与盐水对照相比，杆菌肽和Clor-pactin暴露对细胞活力的影响较小。这项体外研究提供了深入了解灌溉剂对人体细胞的影响，并为进行体内研究提供了必要的基础。我们的发现引起了人们的关注，即某些冲洗溶液可能会对伤口愈合和健康的细胞反应产生负面影响。
UNASSIGNED：对以下条件进行了两次独立实验，重复6次：对照（盐水），杆菌肽,Clorpactin,Irrisept,还有Bactisure.将人成纤维细胞片暴露于这些溶液（1或2分钟），然后用热盐水洗三次。然后将细胞片培养另外的5天和7天的后处理。使用alamarBlue(AB)测定法测量细胞活力。冲洗剂的细胞毒性越大，AB降低越低。
未经批准：对于1分钟的曝光时间，在Clorpactin中注意到AB降低的显着差异，Irrisept,和Bactisure组在治疗后5天（Clorpactinp=0.0003，Irriseptp=7.31×10-15，Bactisurep=6.86×10-14）和7天（所有组p<0.0001）与对照组相比。2分钟暴露组的结果相似。与对照相比,杆菌肽处理的成纤维细胞在所有测量时间均未显示显著差异。
UNASSIGNED:冲洗溶液暴露对细胞活力的影响是不同的。即使短暂暴露（1分钟），Irrisept和Bactisure也显示出最高的细胞毒性，与盐水对照相比，杆菌肽和Clor-pactin暴露对细胞活力的影响较小。这项体外研究提供了深入了解灌溉剂对人体细胞的影响，并为进行体内研究提供了必要的基础。我们的发现引起了人们的关注，即某些冲洗溶液可能会对伤口愈合和健康的细胞反应产生负面影响。
