Abstract：
Glioblastoma multiform (GBM) is an invasive cancer that causes high mortality in patients. Disruption of the apoptosis process is one of the main pathogenesis of the disease. Recently, LncRNAs and miRNAs have been shown to play an important role in the process of apoptosis. To follow the aim of study, 100 patients participated in the two groups of 50 individuals, including 50 GBM patients and 50 healthy individuals as the control group. Mononuclear cells were isolated from peripheral blood samples and RNA extraction was done. The expression changes of miR-17-5p, miR-20-5p, LINC01605, FAS-AS1, and Caspase 3 were examined using RT-PCR in both groups. Expression of LINC01605, miR-20-5p, and miR-17-5p increased in patients, while Caspase 3 and FAS-AS1 decreased; the difference was statistically significant between the two groups. In addition, it was found that these factors have the appropriate sensitivity and specificity as diagnostic markers. Finally, It is suggested to use the LINC01605, FAS-AS1, miR-20-5p, miR-17-5p, and Caspase 3 as apoptosis predictors in the GM patients.
摘要：
多形性胶质母细胞瘤（GBM）是一种侵袭性癌症，可导致患者高死亡率。细胞凋亡进程的中断是该病的主要病发机制之一。最近,LncRNAs和miRNAs已被证明在细胞凋亡过程中起着重要作用。为了遵循学习的目的，100名患者参加了两组50人，包括50例GBM患者和50例健康个体作为对照组。从外周血样品中分离单核细胞并进行RNA提取。miR-17-5p的表达变化,miR-20-5p,在两组中使用RT-PCR检测LINC01605、FAS-AS1和Caspase3。LINC01605,miR-20-5p,miR-17-5p在患者中增加，Caspase3和FAS-AS1降低,两组间差异有统计学意义。此外,发现这些因素作为诊断标志物具有适当的敏感性和特异性。最后,建议使用LINC01605，FAS-AS1，miR-20-5p，miR-17-5p,和Caspase3作为GM患者的凋亡预测因子。
