关键词: Antioxidant Fructan Inulin Polysaccharide Prebiotic

来  源:   DOI:10.1007/s13197-022-05619-6   PDF(Pubmed)

Abstract:
Inulin is the polysaccharide obtained from different plant sources i.e. Wheat, Chicory, Jerusalem artichoke and Dahlia. In this study, Jicama (Pachyrhizus erosus) is used to isolate inulin using the microwave heating. The 1H NMR study reveals the presence of fructose and glucose unit which is the backbone of inulin. Further FT-IR and Raman confirmed the functional groups present in inulin. The UV-Vis spectroscopy analysis depicts the purity of the isolated inulin. The shape and size of the extracted inulin was determined from scanning electron microscopy and dynamic light scattering appeared as flat-flakes and 135 nm respectively. X-ray diffractogram showed semi-crystalline nature suggesting the stability of the extracted inulin. The isolated inulin has phenolic and flavonoid content of 8.1804 ± 6.26 mg gallic acid equivalent/g and 14.387 ± 4.192 mg rutin equivalent/g of dried polysaccharide respectively. The inhibition percentage of DPPH and FRAP of isolated inulin were found to be 75.74 ± 4.5% and 0.11 ± 0.007 respectively. The isolated inulin promotes the growth of probiotics like Enterococcus faecium (MZ540315) and Lactiplantibacillus plantarum (MZ540317). All the analysis suggest the isolated inulin has good prebiotic potential as the commercially available one. The current study proposes that isolated inulin can be used as a prebiotic in the future.
UNASSIGNED:
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13197-022-05619-6.
摘要:
菊粉是从不同的植物来源获得的多糖,即小麦,菊苣,菊芋和大丽花。在这项研究中,Jicama(Pachyrhizuserosus)用于使用微波加热分离菊粉。1HNMR研究揭示了果糖和葡萄糖单元的存在,其为菊粉的骨架。进一步的FT-IR和拉曼证实了菊粉中存在的官能团。UV-Vis光谱分析描绘了分离的菊粉的纯度。从扫描电子显微镜确定提取的菊粉的形状和大小,动态光散射分别显示为扁平薄片和135nm。X射线衍射图显示半结晶性质,表明提取的菊粉的稳定性。分离的菊粉的酚类和类黄酮含量分别为8.1804±6.26mg没食子酸当量/g和14.387±4.192mg芦丁当量/g干多糖。发现分离的菊粉的DPPH和FRAP的抑制百分比分别为75.74±4.5%和0.11±0.007。分离的菊粉促进益生菌如屎肠球菌(MZ540315)和植物乳杆菌(MZ540317)的生长。所有分析都表明,分离的菊粉与市售菊粉一样具有良好的益生元潜力。目前的研究表明,分离的菊粉可以用作未来的益生元。
UNASSIGNED:
UNASSIGNED:在线版本包含补充材料,可在10.1007/s13197-022-05619-6获得。
公众号