Abstract：
The present study reports on the in vivo application of (Bio)silver nanocomposite formulations (LBPC-AgNCs) on wound healing. Additionally, the present study emphasizes the limited uptake of silver by liver and blood tissues as well as the high viability of PBMCs following external LBPC-AgNCs treatment. The wound closure was monitored via stereoscopic microscope, a localization case study in liver and blood tissue was carried out by (Inductively Coupled Plasma-Mass Spectrometers (ICP/MS), and peripheral blood mononuclear cells (PMBC) viability was determined via flow cytometry technique. The silver formulation was applied externally on the site of the wound infection for a period of ten days. At the beginning of the experiment, a moderate decrease in body weight and atypical behavior was observed. However, during the last period of the experiment, no abnormal mouse behaviors were noticed. The wound-healing process took place in a gradual manner, presenting the regeneration effect at around 30% from the fourth day. From the seventh day, the wounds treated with the silver formulation showed 80% of the wound healing potential. The viability of PBMCs was found to be 97%, whereas the concentrations of silver in the liver and blood samples were determined to be 0.022 µg/g and 9.3 µg/g, respectively. Furthermore, the present report becomes a pilot study in transferring from in vitro to in vivo scale (e.g., medical field application) once LBPC-AgNCs have demonstrated a unique wound healing potential as well as a non-toxic effect on the liver and blood.
摘要：
本研究报道了（Bio）银纳米复合材料制剂（LBPC-AgNCs）在伤口愈合中的体内应用。此外,本研究强调肝脏和血液组织对银的吸收有限，以及外部LBPC-AgNCs治疗后PBMC的高活力。通过立体显微镜监测伤口闭合，通过（电感耦合等离子体质谱仪（ICP/MS），通过流式细胞术技术测定外周血单个核细胞（PMBC）的活力。在伤口感染部位外部施用银制剂10天的时间。在实验开始时,观察到体重和非典型行为的中度下降。然而,在实验的最后阶段，没有发现异常的小鼠行为。伤口愈合过程是渐进的，从第四天开始，再生效果在30%左右。从第七天开始，用银制剂处理的伤口显示80%的伤口愈合潜力。PBMC的活力为97%，而肝脏和血液样本中的银浓度分别为0.022µg/g和9.3µg/g，分别。此外，本报告成为从体外转移到体内规模的初步研究(例如，医学领域应用）一旦LBPC-AgNCs证明了独特的伤口愈合潜力以及对肝脏和血液的无毒作用。
