Abstract：
Feeding is crucial for the growth and survival of animals, including humans, but relatively little is known about how it is regulated. Here, we show that larval feeding in Ostrinia furnacalis is regulated by neuropeptide F (NPF, the homologous peptide of mammalian NPY) via the insulin signalling pathway in the midgut. Furthermore, the genes pi3k and mtor in the insulin pathway positively regulate α-amylase and lipase of the midgut by recruiting the transcription factors c-Myc and PPARγ for binding to the promotors of these two enzymes. Importantly, we find that the feeding behaviour and the digestive system of midgut in O. furnacalis larvae are closely related and interactive in that knocking down α-amylase or lipase induces a reduction in larval feeding, while food-deprived larvae lead to fewer expressions of α-amylase and lipase. Importantly, it is the gut NPF that regulates the α-amylase and lipase, while variations of α-amylase and lipase may feed back to the brain NPF. This current study reveals a molecular feedback mechanism between feeding behaviour and the digestive system that is regulated by the conserved NPF via insulin signalling systems in the midgut of O. furnacalis larvae.
摘要：
喂养对动物的生长和生存至关重要，包括人类,但人们对它是如何被监管的知之甚少。这里,我们表明，Ostriniafurnacalis的幼虫摄食受神经肽F（NPF，哺乳动物NPY的同源肽）通过中肠中的胰岛素信号通路。此外，胰岛素途径中的pi3k和mtor基因通过募集转录因子c-Myc和PPARγ与这两种酶的启动子结合来正向调节中肠的α-淀粉酶和脂肪酶。重要的是,我们发现O.furacalis幼虫中肠的摄食行为和消化系统密切相关，相互作用，因为敲除α-淀粉酶或脂肪酶会导致幼虫摄食减少，而缺乏食物的幼虫导致α-淀粉酶和脂肪酶的表达减少。重要的是,它是肠道NPF调节α-淀粉酶和脂肪酶，而α-淀粉酶和脂肪酶的变化可能会反馈到大脑NPF。当前的研究揭示了摄食行为与消化系统之间的分子反馈机制，该机制由保守的NPF通过O.furacalis幼虫中肠的胰岛素信号系统调节。
