Abstract：
The study was conducted to evaluate the effects of 25(OH)VD3 with different inclusion levels of 0, 25, 50 and 75 μg/kg in the diet on growth performance, nutrient digestibility, bone properties and pork quality in growing-finishing pigs. The results showed that the average daily gain (p < 0.05) and body weight (p < 0.10) of pigs showed a trend of increasing quadratically as inclusion levels of 25(OH)VD3 increased. Dietary supplementation of 50 μg/kg 25(OH)VD3 increased calcium digestibility compared with the 0 μg/kg group (p < 0.05), and calcium and phosphorus digestibility increased quadratically as inclusion levels of 25(OH)VD3 increased (p < 0.05). Dietary supplementation of 50 μg/kg 25(OH)VD3 increased concentrations of polyunsaturated fatty acids, and decreased contents of saturated and monounsaturated fatty acids in the longissimus dorsi of pigs (p < 0.05). The addition of 25, 50 and 75 μg/kg 25(OH)VD3 to the diet increased breaking strength and bone stiffness in the tibia compared with the 0 μg/kg group (p < 0.05). Dietary supplementation of 50 μg/kg 25(OH)VD3 improved the activities of superoxide dismutase (SOD) and catalase (CAT), and increased the messenger RNA (mRNA) expression of Cu/Zn SOD in the longissimus dorsi compared with the 0 μg/kg group (p < 0.05). Supplementing 50 μg/kg 25(OH)VD3 improved the mRNA expression of calcium-binding protein D9k (CaBP-D9k) and D28k (CaBP-D28K) in the liver compared with the 0 μg/kg 25(OH)D3 group (p < 0.05). In conclusion, a diet with an added dose of 50 μg/kg 25(OH)VD3 showed a greatest growth performance of growing-finishing pigs, and 25(OH)VD3 enhanced calcium deposition and antioxidant capacity in the longissimus dorsi, which may be associated with improved expression of calcium ion channel proteins.
摘要：
该研究旨在评估日粮中不同包含水平为0、25、50和75μg/kg的25（OH）VD3对生长性能的影响，营养素消化率，生长肥育猪的骨骼特性和猪肉品质。结果表明，随着25（OH）VD3含量的增加，猪的平均日增重（p＆lt;0.05）和体重（p＆lt;0.10）呈二次增加趋势。与0μg/kg组相比，日粮补充50μg/kg25（OH）VD3可增加钙消化率（p<0.05），随着25（OH）VD3包涵体水平的增加，钙和磷的消化率呈二次增加（p＆lt;0.05）。饮食补充50μg/kg25（OH）VD3可增加多不饱和脂肪酸的浓度，猪背最长肌中饱和脂肪酸和单不饱和脂肪酸含量降低(p<0.05)。与0μg/kg组相比，饮食中添加25、50和75μg/kg25（OH）VD3可增加胫骨的断裂强度和骨刚度（p＆lt;0.05）。日粮补充50μg/kg25(OH)VD3可提高超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性,与0μg/kg组相比，背最长肌Cu/ZnSOD的信使RNA（mRNA）表达增加（p<0.05）。与0μg/kg25（OH）D3组相比，补充50μg/kg25（OH）VD3可提高肝脏中钙结合蛋白D9k（CaBP-D9k）和D28k（CaBP-D28K）的mRNA表达（p＆lt;0.05）。总之,添加剂量为50μg/kg25（OH）VD3的饮食显示出生长肥育猪的最大生长性能，25(OH)VD3增强了背最长肌的钙沉积和抗氧化能力，这可能与钙离子通道蛋白表达的改善有关。
