Abstract：
A 10-year-old female Golden Retriever was presented for a recheck after the complete removal of low-grade complex mammary carcinoma. The in-house ProCyte Dx automated counts revealed moderate regenerative anemia and moderate eosinophilia. The ProCyte Dx WBC scattergram showed a cloud in an unusual place parallel and to the right of the monocyte dot plot location. Cells were classified as either monocytes or neutrophils with no clear separation. Complete blood count analysis performed in the laboratory on a Sysmex XT-2000iV analyzer showed moderate regenerative anemia and WBC count within RI; a differential count was not provided by the instrument. On the Sysmex XT-2000iV DIFF scattergram, neutrophil and eosinophil dot plots were present at the respective locations and appeared separated, but the instrument did not provide numerical results. In addition to the normal lymphocyte dot plot location, the second cloud of cells classified as lymphocytes was displayed to the right of the monocyte dot plot area. On the WBC/BASO scattergram, the second population of cells was present above and to the right of the leukocyte cluster. Morphologic assessment of the blood smear detected mastocytemia with 16% poorly granulated and degranulated mast cells. FNAs from the liver and spleen contained large aggregates of poorly granulated mast cells. C-kit somatic mutation screening detected the presence of point mutation S479I in exon 9 of the canine c-KIT gene. This is the first description of abnormal scattergrams from ProCyte Dx and Sysmex XT-2000iV analyzers in a dog with concurrent mastocytemia and systemic mastocytosis, and where cytologic assessments of a blood smear, liver, and spleen, and c-kit somatic mutation analysis were performed.
摘要：
在完全切除低度复杂的乳腺癌后，一名10岁的雌性金毛被提出进行重新检查。内部ProCyteDx自动计数显示中度再生性贫血和中度嗜酸性粒细胞增多。ProCyteDxWBC散点图显示，在单核细胞点图位置右侧平行的不寻常位置处有云。细胞被分类为单核细胞或嗜中性粒细胞,没有明确的分离。在实验室中在SysmexXT-2000iV分析仪上进行的全血细胞计数分析显示中度再生性贫血和RI内的WBC计数;仪器未提供差异计数。在SysmexXT-2000iVDIFF散点图上，嗜中性粒细胞和嗜酸性粒细胞点图出现在各自的位置，并出现分离，但仪器没有提供数值结果。除了正常的淋巴细胞点图位置，分类为淋巴细胞的第二细胞云显示在单核细胞点图区域的右侧.在WBC/BASO散点图上，第二个细胞群出现在白细胞簇的上方和右侧。血液涂片的形态学评估检测到的肥大细胞有16％的颗粒化和去粒化的肥大细胞。来自肝脏和脾脏的FNA含有大量颗粒状差的肥大细胞聚集体。C-kit体细胞突变筛查检测到犬c-KIT基因外显子9中存在点突变S479I。这是ProCyteDx和SysmexXT-2000iV分析仪在并发肥大细胞和系统性肥大细胞增多症的狗中异常散点图的首次描述，血液涂片的细胞学评估，肝脏,和脾脏,和c-kit体细胞突变分析。
