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Abstract：
Toxoplasmosis is a zoonotic disease caused by the obligate intracellular protozoan parasite T. gondii which is widely prevalent in humans and animals worldwide. The diagnosis of toxoplasmosis and distinguishing acute or chronic T. gondii infections have utmost importance for humans and animals. The TgSAG1, TgGRA7, and TgBAG1 proteins were used in the present study to develop the serological rSAG1-ELISA, rGRA7-ELISA and rBAG1-ELISA methods for the testing of T. gondii specific IgG and IgM antibodies and differentiating acute or chronic toxoplasmosis in 3733 animals, including Tibetan sheep, yaks, pigs, cows, cattle, horses, chickens, camels and donkeys from the Qinghai-Tibetan Plateau. The ELISA tests showed that the overall positivity of IgG antibody was 21.1% (786/3733), 15.3% (570/3733) and 18.2% (680/3733) for rSAG1-, rGRA7- and rBAG1-ELISA, respectively, and the positivity of IgM antibody was 11.8% (439/3733), 13.0% (486/3733) and 11.8% (442/3733) for rSAG1-, rGRA7- and rBAG1-ELISA, respectively. A total of 241 animals (6.5%) positive for all rSAG1-, rGRA7- and rBAG1-IgG were found in this study, and the 141 animals (3.8%) tested were anti-T. gondii IgM positive in all three ELISAs. Moreover, the 338, 284 and 377 animals were IgG positive in rSAG1 + rGRA7-, rBAG1 + rGRA7- and rSAG1 + rBAG1- ELISAs respectively, and the 346, 178 and 166 animals in rSAG1 + rGRA7-, rBAG1 + rGRA7- and rSAG1 + rBAG1-ELISAs were IgM positive respectively. The results confirmed that the application of SAG1, GRA7, and BAG1 recombinant antigens could successfully be used in the detection of specific IgG and IgM antibodies for distinguishing between acute or chronic T. gondii infections. It is inferred that the forms in which current animal species in the plateau area were infected with T. gondii, and the period of infection or the clinical manifestations of the current infections may be different. The present study provides substantial clinical evidence for the differential diagnosis of toxoplasmosis, and the classification of acute and chronic T. gondii infections.
摘要：
弓形虫病是由专性细胞内原生动物寄生虫弓形虫引起的一种人畜共患疾病，在全球范围内广泛流行。弓形虫病的诊断和区分急性或慢性弓形虫感染对人类和动物至关重要。TgSAG1，TgGRA7和TgBAG1蛋白在本研究中用于开发血清学rSAG1-ELISA，rGRA7-ELISA和rBAG1-ELISA方法，用于测试弓形虫特异性IgG和IgM抗体并区分3733只动物的急性或慢性弓形虫病，包括西藏羊,牦牛,猪,奶牛,牛,马,鸡,来自青藏高原的骆驼和驴。ELISA检测显示IgG抗体的总体阳性率为21.1%(786/3733),rSAG1-15.3%(570/3733)和18.2%(680/3733)，rGRA7-和rBAG1-ELISA,分别,IgM抗体阳性率为11.8%(439/3733),rSAG1-13.0%(486/3733)和11.8%(442/3733)，rGRA7-和rBAG1-ELISA,分别。共有241只动物（6.5％）对所有rSAG1-呈阳性，在这项研究中发现了rGRA7-和rBAG1-IgG，测试的141只动物（3.8％）是抗T。所有三个ELISA中的gondiiIgM阳性。此外，338、284和377只动物在rSAG1+rGRA7-中呈IgG阳性，rBAG1+rGRA7-和rSAG1+rBAG1-ELISA，rSAG1+rGRA7-中的346、178和166只动物，rBAG1+rGRA7-和rSAG1+rBAG1-ELISA分别为IgM阳性。结果证实，应用SAG1、GRA7和BAG1重组抗原可成功用于特异性IgG和IgM抗体的检测，以区分急性或慢性弓形虫感染。据推断，高原地区当前动物物种感染弓形虫的形式，感染时期或当前感染的临床表现可能不同。本研究为弓形虫病的鉴别诊断提供了大量的临床证据。以及急性和慢性弓形虫感染的分类。
