PDF(Pubmed)
Abstract：
UNASSIGNED: Akt, also known as protein kinase B, is a serine/threonine-specific protein-kinase which plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, transcription and cell migration. The activation of Akt is one of the most frequent alterations observed in human cancer and tumour cells. Akt regulates cellular survival and metabolism by binding and regulating many downstream effectors, e.g., Nuclear Factor-kB, murine double minute 2(MDM2).
UNASSIGNED: To evaluate and compare immunohistochemical expression of Akt in normal epithelium and different histological grades of epithelial dysplasias.
UNASSIGNED: Forty paraffin-embedded tissue sections were used for the immunohistochemical study of which 10 cases of normal epithelium, 10 cases of each mild, moderate and severe epithelial dysplasia which were diagnosed by haematoxylin and eosin procedures. The tissue sections were immunohistochemically analysed for expression of Akt in different grades of epithelial dysplasia by using anti-Akt-1 monoclonal antibody. Statistical analysis was carried out using statistical package for social science (SPSS, V 10.5). The data were analysed using Chi-square test and P < 0.05 was considered statistically significant.
UNASSIGNED: An overall significant difference was observed when normal tissues were compared with epithelial dysplasia with a Chi-square value of 21.04, but there was no statistical significance between the three grades of epithelial dysplasias. In conclusion, this study suggests that Akt-1 overexpression can be one of the useful diagnostic markers for predicting the potential behaviour of oral dysplasias transforming into oral squamous cell carcinoma (OSCC).
UNASSIGNED: To evaluate and compare immunohistochemical expression of Akt in normal epithelium and different histological grades of epithelial dysplasias.
UNASSIGNED: Forty paraffin-embedded tissue sections were used for the immunohistochemical study of which 10 cases of normal epithelium, 10 cases of each mild, moderate and severe epithelial dysplasia which were diagnosed by haematoxylin and eosin procedures. The tissue sections were immunohistochemically analysed for expression of Akt in different grades of epithelial dysplasia by using anti-Akt-1 monoclonal antibody. Statistical analysis was carried out using statistical package for social science (SPSS, V 10.5). The data were analysed using Chi-square test and P < 0.05 was considered statistically significant.
UNASSIGNED: An overall significant difference was observed when normal tissues were compared with epithelial dysplasia with a Chi-square value of 21.04, but there was no statistical significance between the three grades of epithelial dysplasias. In conclusion, this study suggests that Akt-1 overexpression can be one of the useful diagnostic markers for predicting the potential behaviour of oral dysplasias transforming into oral squamous cell carcinoma (OSCC).
摘要：
未经批准：Akt，也被称为蛋白激酶B，是一种丝氨酸/苏氨酸特异性蛋白激酶，在葡萄糖代谢等多种细胞过程中起关键作用，凋亡，转录和细胞迁移。Akt的激活是在人类癌症和肿瘤细胞中观察到的最常见的改变之一。Akt通过结合和调节许多下游效应子调节细胞存活和代谢，例如，核因子-kB,鼠双分钟2（MDM2）。
UNASSIGNED：评估和比较Akt在正常上皮和不同组织学分级上皮发育不良中的免疫组织化学表达。
UNASSIGNED：使用40个石蜡包埋的组织切片进行免疫组织化学研究，其中10例正常上皮，每个轻度10例，通过苏木精和伊红程序诊断的中度和重度上皮异型增生。通过使用抗Akt-1单克隆抗体对组织切片进行免疫组织化学分析，以在不同级别的上皮异型增生中Akt的表达。使用社会科学统计软件包进行统计分析(SPSS，V10.5)。数据分析采用卡方检验，P<0.05为统计学意义。
UNASSIGNED：当正常组织与上皮异型增生比较时，观察到总体显着差异，卡方值为21.04，但上皮异型增生的三个等级之间没有统计学意义。总之,这项研究表明,Akt-1过表达可能是预测口腔发育不良转化为口腔鳞状细胞癌(OSCC)的潜在行为的有用诊断标志物之一.
UNASSIGNED：评估和比较Akt在正常上皮和不同组织学分级上皮发育不良中的免疫组织化学表达。
UNASSIGNED：使用40个石蜡包埋的组织切片进行免疫组织化学研究，其中10例正常上皮，每个轻度10例，通过苏木精和伊红程序诊断的中度和重度上皮异型增生。通过使用抗Akt-1单克隆抗体对组织切片进行免疫组织化学分析，以在不同级别的上皮异型增生中Akt的表达。使用社会科学统计软件包进行统计分析(SPSS，V10.5)。数据分析采用卡方检验，P<0.05为统计学意义。
UNASSIGNED：当正常组织与上皮异型增生比较时，观察到总体显着差异，卡方值为21.04，但上皮异型增生的三个等级之间没有统计学意义。总之,这项研究表明,Akt-1过表达可能是预测口腔发育不良转化为口腔鳞状细胞癌(OSCC)的潜在行为的有用诊断标志物之一.
