Abstract：
Advancements in lymphography technology are essential for comprehensive investigation of the lymphatic system and its function. Here, a shortwave infrared (SWIR) luminescence imaging of lymphatic vessels is proposed in both normal and lymphatic dysfunction in rat models with PbS quantum dots (PbS Qdots). The lymphography with PbS Qdots can clearly and rapidly demonstrate the normal lymphatic morphology in both the tail and hind limb. More importantly, compared to ICG, SWIR luminescence imaging with PbS Qdots can easily identify the dominant lymphatic vessel and node with higher luminescence signal in rats. Moreover, lymphatic pump is identified as segment contracting sections with a size of ≈1 cm in rat by in vivo SWIR lymphograhy, which propose a direct feature for precise evaluation of lymphatic function. Notably, in vivo SWIR luminescence imaging with PbS Qdots also clearly deciphers the in vivo pattern of morphological and function recovery from lymphatic system in rat model. In summary, SWIR luminescence imaging with PbS Qdots can improve the lymphography and thus deepen the understanding of the morphology and structure of the lymphatic system as well as lymphatic function such as lymphatic pump, which will facilitate the diagnosis of lymphatic dysfunction in the future.
摘要：
淋巴造影技术的进步对于全面研究淋巴系统及其功能至关重要。这里,在使用PbS量子点（PbSQdots）的大鼠模型中，在正常和淋巴功能障碍中都提出了淋巴管的短波红外（SWIR）发光成像。PbSQdots的淋巴造影可以清楚，快速地显示尾巴和后肢的正常淋巴形态。更重要的是,与ICG相比，用PbSQdots进行SWIR发光成像可以很容易地识别大鼠中具有较高发光信号的优势淋巴管和结节。此外，通过体内SWIR淋巴吸收，将淋巴泵鉴定为大鼠大小约1cm的节段收缩切片，提出了精确评估淋巴功能的直接特征。值得注意的是,使用PbSQdots的体内SWIR发光成像还可以清楚地破译大鼠模型中淋巴系统的形态和功能恢复的体内模式。总之,用PbSQdots进行SWIR发光成像可以改善淋巴造影，从而加深对淋巴系统的形态和结构以及淋巴泵等淋巴功能的了解。这将有助于未来淋巴功能障碍的诊断。
