Abstract：
BACKGROUND: The nano-sized, lipid bilayer-delimited placental extracellular vesicles (PEVs) released by the placenta are now regarded as important mediators involved in various physiological and pathological processes of pregnant women. The number and contents of PEVs are significantly altered in preeclampsia and are considered as potential biomarkers. However, the distribution pattern of PEVs in the maternal circulation in different pregnancy status is still unclear for the limitation of the traditional method with low sensitivity.
METHODS: In this work, we recruited 561 pregnant women with different pregnancy status and investigated the distribution pattern of PEVs in the maternal circulation based on a single extracellular vesicle analysis method and placental alkaline phosphatase (PLAP), a placenta-specific marker.
RESULTS: The concentration of PEVs in pregnant women increased with the progression of gestational age, while the ratio of PEVs decreased to about 10% in the third trimester. Surprisingly, the PLAP+ EVs also presented in the plasma of non-pregnant women and normal male about 5%. The change in the ratio of PEVs can reflect the pregnancy status and also had a better diagnostic value in severe preeclampsia (AUC = 0.7811).
CONCLUSIONS: Our study not only reveals the distribution pattern of PEVs, but also identifies the diagnostic potential of PEVs as biomarkers.
METHODS: In this work, we recruited 561 pregnant women with different pregnancy status and investigated the distribution pattern of PEVs in the maternal circulation based on a single extracellular vesicle analysis method and placental alkaline phosphatase (PLAP), a placenta-specific marker.
RESULTS: The concentration of PEVs in pregnant women increased with the progression of gestational age, while the ratio of PEVs decreased to about 10% in the third trimester. Surprisingly, the PLAP+ EVs also presented in the plasma of non-pregnant women and normal male about 5%. The change in the ratio of PEVs can reflect the pregnancy status and also had a better diagnostic value in severe preeclampsia (AUC = 0.7811).
CONCLUSIONS: Our study not only reveals the distribution pattern of PEVs, but also identifies the diagnostic potential of PEVs as biomarkers.
摘要：
背景：纳米尺寸，由胎盘释放的脂质双层界定的胎盘细胞外囊泡（PEV）现在被认为是参与孕妇各种生理和病理过程的重要介质。PEV的数量和含量在先兆子痫中显著改变并且被认为是潜在的生物标志物。然而,由于传统方法敏感性低的局限性，不同妊娠状态下母体循环中PEV的分布规律尚不清楚。
方法：在这项工作中，我们招募了561名不同妊娠状态的孕妇，并根据单一的细胞外囊泡分析方法和胎盘碱性磷酸酶（PLAP）调查了PEV在母体循环中的分布模式，胎盘特异性标记物。
结果：孕妇体内的PEV浓度随着胎龄的增加而增加，而PEV的比率在妊娠晚期降至约10%。令人惊讶的是,PLAP+EV也存在于非孕妇和正常男性的血浆中,约5%.PEV比值的变化可以反映妊娠状态,对重度子痫前期也有较好的诊断价值(AUC=0.7811)。
结论：我们的研究不仅揭示了PEV的分布模式，而且还确定了PEV作为生物标志物的诊断潜力。
方法：在这项工作中，我们招募了561名不同妊娠状态的孕妇，并根据单一的细胞外囊泡分析方法和胎盘碱性磷酸酶（PLAP）调查了PEV在母体循环中的分布模式，胎盘特异性标记物。
结果：孕妇体内的PEV浓度随着胎龄的增加而增加，而PEV的比率在妊娠晚期降至约10%。令人惊讶的是,PLAP+EV也存在于非孕妇和正常男性的血浆中,约5%.PEV比值的变化可以反映妊娠状态,对重度子痫前期也有较好的诊断价值(AUC=0.7811)。
结论：我们的研究不仅揭示了PEV的分布模式，而且还确定了PEV作为生物标志物的诊断潜力。
