The vaginal fungi of vaginal discharge were detected by fluorescence method, i.e., by liquid-based thin-layer fungi fluorescence morphology staining detection kit (liquid-based fungal method), saline smear method and fungal culture method.
The positive rate of liquid-based fungal method, saline smear method was 50%, 25.75% respectively. The positive rate of liquid-based fungal method were 50%. The true positive rate of liquid-based fungal method (87.85%) was higher than that of saline smear method (45.79%, P < 0.001), which was easy to miss diagnosis. Moreover, the Kappa (K) of liquid-based fungal method was 0.81, and P < 0.01, which was statistically significant, indicating that the consistency of the two detection methods is good. Of the eight common symptoms of fungal vaginitis, the positive symptom coincidence rate of liquid-based fungal method was consistent with that of fungal culture method. It was also easier to see fungi under a microscope than with saline smear method.
The liquid-based fungal method has a high positive coincidence rate and accuracy in the detection of vaginal fungi, and it is convenient to operate and implement steps. Therefore, it may be applied in clinical practice. Or a combination of several detection methods can be used.
方法:采用荧光法检测阴道分泌物中的真菌,即,通过液基薄层真菌荧光形态学染色检测试剂盒(液基真菌法),生理盐水涂片法和真菌培养法。
结果:液基真菌法的阳性率,盐水涂片方法为50%,分别为25.75%。液基真菌法的阳性率为50%。液基真菌法的真阳性率(87.85%)高于盐水涂片法(45.79%,P<0.001),很容易漏诊.此外,液基真菌法的Kappa(K)为0.81,P<0.01,表明两种检测方法的一致性较好。在真菌性阴道炎的八种常见症状中,液基真菌法阳性症状符合率与真菌培养法一致。与盐水涂片法相比,在显微镜下更容易看到真菌。
结论:液基真菌法在阴道真菌检测中具有较高的阳性符合率和准确性,操作和实施步骤方便。因此,可应用于临床。或者可以使用几种检测方法的组合。