Abstract：
BACKGROUND: Currently, no recognized evidence is known about the bacterial communities found within seminal vesicles (SV) of men presenting with refractory hematospermia.
RESULTS: Fifteen male patients with refractory hematospermia or anejaculation were enrolled, and 15 SV-Infection (SV-In) samples from SV with hemorrhage and/or stones, 11 SV-Control (SV-C) samples from SV with non-infection, and 14 Urine (Urine) samples from posterior urethra were obtained via transurethral seminal vesiculoscopy. Then the high-throughput 16 S rRNA gene sequencing method was performed to characterize the microbiota profile. Finally, a total of 1535 operational taxonomic units (OTUs) were found, 1295 OTUs were shared across three groups, 7 OTUs, 45 OTUs, and 48 OTUs were unique to SV-C group, SV-In group, and Urine group, respectively. The 5 top bacterial phyla (mean relative abundance) in all samples were Firmicutes (52.08%), Bacteroidetes (21.69%), Proteobacteria (12.72%), Actinobacteria (9.64%), and Fusobacteria (1.62%), the 5 top bacterial genera in all samples were Bacteroides (9.13%), Lactobacillus (5.38%), Bifidobacterium (5.35%), Faecalibacterium (5.10%), and Allobaculum (3.34%), of which Bifidobacterium had the highest level in SV-C samples and had a significant difference (P < 0.05) across all groups. Differential analysis showed genera Leuconostoc and LachnospiraceaeFCS020group were identified as biomarkers in the SV-In microbiota.
CONCLUSIONS: Altered microbiota composition in seminal vesicles is related to refractory hematospermia in men, and the distribution of genus Leuconostoc or LachnospiraceaeFCS020group within seminal vesicles may interact with hematospermia. This study provides clues for the diagnosis and treatment of this urologic disorder.
RESULTS: Fifteen male patients with refractory hematospermia or anejaculation were enrolled, and 15 SV-Infection (SV-In) samples from SV with hemorrhage and/or stones, 11 SV-Control (SV-C) samples from SV with non-infection, and 14 Urine (Urine) samples from posterior urethra were obtained via transurethral seminal vesiculoscopy. Then the high-throughput 16 S rRNA gene sequencing method was performed to characterize the microbiota profile. Finally, a total of 1535 operational taxonomic units (OTUs) were found, 1295 OTUs were shared across three groups, 7 OTUs, 45 OTUs, and 48 OTUs were unique to SV-C group, SV-In group, and Urine group, respectively. The 5 top bacterial phyla (mean relative abundance) in all samples were Firmicutes (52.08%), Bacteroidetes (21.69%), Proteobacteria (12.72%), Actinobacteria (9.64%), and Fusobacteria (1.62%), the 5 top bacterial genera in all samples were Bacteroides (9.13%), Lactobacillus (5.38%), Bifidobacterium (5.35%), Faecalibacterium (5.10%), and Allobaculum (3.34%), of which Bifidobacterium had the highest level in SV-C samples and had a significant difference (P < 0.05) across all groups. Differential analysis showed genera Leuconostoc and LachnospiraceaeFCS020group were identified as biomarkers in the SV-In microbiota.
CONCLUSIONS: Altered microbiota composition in seminal vesicles is related to refractory hematospermia in men, and the distribution of genus Leuconostoc or LachnospiraceaeFCS020group within seminal vesicles may interact with hematospermia. This study provides clues for the diagnosis and treatment of this urologic disorder.
摘要：
背景：目前，尚无公认的证据表明，在患有难治性血精子症的男性精囊（SV）内发现了细菌群落。
结果：纳入15例男性难治性血精症或射精患者，和15个SV感染（SV-In）样本，包括出血和/或结石，11个来自非感染SV的SV对照（SV-C）样品，通过经尿道精囊镜从后尿道获得14个尿液（尿液）样本。然后进行高通量16SrRNA基因测序方法以表征微生物区系谱。最后,共发现1535个操作分类单位(OTU)，1295个OTU在三组之间共享，7个OTU，45个OTU,48个OTU是SV-C组独有的，SV-In组,尿组，分别。所有样品中5个最高的细菌门（平均相对丰度）是Firmicutes（52.08％），拟杆菌(21.69%),变形杆菌（12.72%），放线菌(9.64%),和镰刀菌(1.62%)，所有样本中排名前5位的细菌属为拟杆菌(9.13%)，乳酸菌(5.38%),双歧杆菌（5.35%），粪杆菌(5.10%),和Allobaculum(3.34%)，其中双歧杆菌在SV-C样品中的水平最高，并且在所有组之间具有显着差异（P<0.05）。差异分析显示明串珠菌属和LachnospiaceaFCS020组被鉴定为SV-In微生物群中的生物标志物。
结论：精囊菌群组成的改变与男性难治性血精有关，精囊内明串珠菌属或LachnospienceFCS020组的分布可能与血精相互作用。本研究为该泌尿系疾病的诊断和治疗提供线索。
结果：纳入15例男性难治性血精症或射精患者，和15个SV感染（SV-In）样本，包括出血和/或结石，11个来自非感染SV的SV对照（SV-C）样品，通过经尿道精囊镜从后尿道获得14个尿液（尿液）样本。然后进行高通量16SrRNA基因测序方法以表征微生物区系谱。最后,共发现1535个操作分类单位(OTU)，1295个OTU在三组之间共享，7个OTU，45个OTU,48个OTU是SV-C组独有的，SV-In组,尿组，分别。所有样品中5个最高的细菌门（平均相对丰度）是Firmicutes（52.08％），拟杆菌(21.69%),变形杆菌（12.72%），放线菌(9.64%),和镰刀菌(1.62%)，所有样本中排名前5位的细菌属为拟杆菌(9.13%)，乳酸菌(5.38%),双歧杆菌（5.35%），粪杆菌(5.10%),和Allobaculum(3.34%)，其中双歧杆菌在SV-C样品中的水平最高，并且在所有组之间具有显着差异（P<0.05）。差异分析显示明串珠菌属和LachnospiaceaFCS020组被鉴定为SV-In微生物群中的生物标志物。
结论：精囊菌群组成的改变与男性难治性血精有关，精囊内明串珠菌属或LachnospienceFCS020组的分布可能与血精相互作用。本研究为该泌尿系疾病的诊断和治疗提供线索。
