Abstract：
Fucoidans are fucose rich sulfated polysaccharides that are found in the cell wall of brown seaweeds and have been shown to have several beneficial bioactivities. In the present study, we report a new enzymatic extraction technique for the production of pure and intact fucoidans from the two brown seaweeds Saccharina latissima and Alaria esculenta. This new extraction protocol uses the commercial cellulase blend Cellic® CTec2 in combination with endo- and exo-acting thermophilic alginate lyases. The fucoidans obtained by this extraction technique are compared to traditionally extracted fucoidans in terms of chemical compositions and molecular weights and are shown to contain significantly higher amounts of fucose and sulfate, the main components of fucoidans, while cellulose, laminarin, and alginate contamination is low. Thus, by using this combination of enzymes, the extracted fucoidans do not undergo depolymerization during extraction and additional purification steps are not needed. The high purity fucoidans isolated by this new enzymatic extraction technique can be used to provide insight into the different fucoidan structures and biological activities.
摘要：
岩藻依聚糖是在褐藻的细胞壁中发现的富含岩藻糖的硫酸化多糖,并且已经显示具有若干有益的生物活性。在本研究中,我们报告了一种新的酶法提取技术，用于从两种棕色海藻中生产纯的和完整的岩藻依聚糖。这种新的提取方案使用商业纤维素酶混合物Cellic®CTec2与内外作用的嗜热藻酸盐裂解酶组合。通过这种提取技术获得的岩藻依聚糖在化学组成和分子量方面与传统提取的岩藻依聚糖进行比较，并显示含有明显更高的岩藻糖和硫酸盐的量。岩藻依聚糖的主要成分，而纤维素，laminarin,藻酸盐污染低。因此，通过使用这种酶的组合，所提取的岩藻依聚糖在提取期间不经历解聚,并且不需要额外的纯化步骤。通过这种新的酶促提取技术分离的高纯度岩藻依聚糖可用于了解不同的岩藻依聚糖结构和生物活性。
