Abstract：
The overuse and improper disposal of antibiotics results in antibiotic resistance. This raises concern over the presence of antibiotic resistant bacteria (ARB) in waterways and pose health risks of antibiotic resistant infections to water recreationists. The purpose of this study was to monitor water quality, microbial ecology, and antibiotic resistance in water and biofilm on submerged plastics at two public boat launches in southeastern Louisiana. Water and biofilm samples were collected once a month, in triplicate, from two public boat launches in Louisiana, USA for a year. Water quality metrics included nitrate, ammonia, sulfate, phosphate, and organic carbon. Water samples were tested for total and fecal coliform abundance and the presence of ARB. Out of 131 bacterial isolates studied from these two sites, 86% of them tested positive for antibiotic resistance with multi-drug resistance. Antibiotic resistance genes (ARGs) for sulfonamide (sul2), bacitracin (bacA) and ampicillin (ampA) were identified in bacterial isolates from water and biofilm samples at both sites. Molecular genetic diversity analysis identified distinct taxonomic diversity differences in biofilm bacteria compared to the planktonic bacteria in the surrounding water. Biofilm samples showed increased diversity at the phylum, genus, and species levels.
摘要：
抗生素的过度使用和不当处置导致抗生素耐药性。这引起了人们对水道中抗生素抗性细菌（ARB）存在的担忧，并对水娱乐者造成抗生素抗性感染的健康风险。这项研究的目的是监测水质，微生物生态学,在路易斯安那州东南部的两个公共船只上，水中的抗生素抗性和水下塑料上的生物膜。每月采集一次水和生物膜样品,一式三份，从路易斯安那州的两艘公共船出发，美国一年。水质指标包括硝酸盐，氨,硫酸盐,磷酸盐,和有机碳。测试水样的总和粪便大肠杆菌丰度以及ARB的存在。在这两个地点研究的131种细菌中，其中86％的抗生素耐药性和多重耐药性呈阳性。磺胺（sul2）的抗生素抗性基因（ARGs），在两个地点的水和生物膜样品中的细菌分离物中鉴定了杆菌肽（bacracin）和氨苄青霉素（ampA）。分子遗传多样性分析确定了与周围水中的浮游细菌相比，生物膜细菌的分类学多样性差异。生物膜样本显示门的多样性增加，属,和物种水平。
