PDF(Pubmed)
Abstract：
Faba bean (Vicia faba L.) is an important food and feed legume crop in the world. The root rot complex caused by various pathogens is a main constraint in faba bean production. In April 2021, a severe disease of faba bean with symptoms of black necrosis on roots occurred in experimental fields at the Linxia Institute of Agricultural Sciences, Gansu Province, China. This study aimed to identify the pathogen and evaluate the resistance of faba bean cultivars. The pathogen was isolated from infected soils, and five representative isolates were identified as Berkeleyomyces rouxiae based on morphological characteristics, pathogenicity, and molecular phylogenetic analyses. A host range test showed that chickpea, common bean, cowpea, mung bean, rice bean, lentil, and hyacinth bean were susceptible hosts of the faba bean isolate, whereas adzuki bean, pea, and soybean were non-susceptible hosts, and maize and wheat were non-hosts. Identification of resistance among 36 faba bean cultivars was carried out, and six cultivars were found to be moderately resistant to B. rouxiae. In this study, we first reported black root rot on faba bean caused by B. rouxiae, confirmed and expanded the host range of B. rouxiae, and identified resistant faba bean cultivars.
摘要：
蚕豆（ViciafabaL.）是世界上重要的食粮豆类作物。各种病原体引起的根腐病复合体是蚕豆生产中的主要制约因素。2021年4月，临夏农业科学研究所的试验田发生了严重的蚕豆疾病，其根部出现黑色坏死症状，甘肃省,中国。本研究旨在鉴定病原菌并评估蚕豆品种的抗性。病原体是从受感染的土壤中分离出来的，并根据形态特征鉴定了5个代表性分离株为鲁氏病菌，致病性，和分子系统发育分析。宿主范围测试表明鹰嘴豆，普通豆，牛仔,绿豆,米豆,扁豆,风信子豆是蚕豆分离株的易感宿主，而小豆，豌豆,大豆是非易感宿主，玉米和小麦是非寄主。对36个蚕豆品种进行了抗性鉴定，并发现六个品种对肉芽胞杆菌具有中等抗性。在这项研究中,我们首先报道了由B.rouxiae引起的蚕豆黑根腐病，证实并扩大了鲁氏芽孢杆菌的寄主范围，并鉴定了抗性蚕豆品种。
